Skip to main content
NCBI home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1995 Apr;59(2):118–123.

Interaction with pig ileal explants of Escherichia coli O45 isolates from swine with postweaning diarrhea.

C Zhu 1, J Harel 1, M Jacques 1, J M Fairbrother 1
PMCID: PMC1263748  PMID: 7648523

Abstract

We have previously observed that Escherichia coli O45 isolates from swine postweaning diarrhea (PWD) induced attaching-effacing (A/E) lesions in experimentally inoculated gnotobiotic piglets. In the present work, ileal explant culture has been used as an in vitro model for the study of the development of A/E lesions due to these isolates. The characteristic intimate bacterial attachment and microvilli effacement with cupping and pedestal formation, identical to that observed in gnotobiotic piglets, was demonstrated in pig ileal explants inoculated with O45 E. coli isolates. The initial attachment of bacteria to the enterocytes was observed from 2 to 4 h postinoculation (PI) and full development of A/E lesions was observed within 8 h PI. In this model, we observed that 22 of 25 eaeA-positive O45 isolates induced A/E lesions. However, A/E lesions were not observed for any of 7 eaeA-negative O45 isolates. Thus, we describe a useful in vitro model for the study of A/E capacity of porcine E. coli. Use of this model has enabled us to demonstrate the relatedness of the eaeA gene to A/E capability among porcine O45 E. coli from PWD.

Full text

PDF
118

Images in this article

120Fig. 1.
on p.120
121Fig. 2.
on p.121

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldini M. M., Kaper J. B., Levine M. M., Candy D. C., Moon H. W. Plasmid-mediated adhesion in enteropathogenic Escherichia coli. J Pediatr Gastroenterol Nutr. 1983;2(3):534–538. doi: 10.1097/00005176-198302030-00023. [DOI] [PubMed] [Google Scholar]
  2. Batt R. M., Embaye H., Hunt J., Hart C. A. Ultrastructural damage to equine intestinal epithelium induced by enteropathogenic Escherichia coli. Equine Vet J. 1989 Sep;21(5):373–375. doi: 10.1111/j.2042-3306.1989.tb02695.x. [DOI] [PubMed] [Google Scholar]
  3. Broes A., Drolet R., Jacques M., Fairbrother J. M., Johnson W. M. Natural infection with an attaching and effacing Escherichia coli in a diarrheic puppy. Can J Vet Res. 1988 Apr;52(2):280–282. [PMC free article] [PubMed] [Google Scholar]
  4. Cantey J. R., Blake R. K. Diarrhea due to Escherichia coli in the rabbit: a novel mechanism. J Infect Dis. 1977 Mar;135(3):454–462. doi: 10.1093/infdis/135.3.454. [DOI] [PubMed] [Google Scholar]
  5. Cantey J. R., Lushbaugh W. B., Inman L. R. Attachment of bacteria to intestinal epithelial cells in diarrhea caused by Escherichia coli strain RDEC-1 in the rabbit: stages and role of capsule. J Infect Dis. 1981 Feb;143(2):219–230. doi: 10.1093/infdis/143.2.219. [DOI] [PubMed] [Google Scholar]
  6. Chanter N., Morgan J. H., Bridger J. C., Hall G. A., Reynolds D. J. Dysentery in gnotobiotic calves caused by atypical Escherichia coli. Vet Rec. 1984 Jan 21;114(3):71–71. doi: 10.1136/vr.114.3.71. [DOI] [PubMed] [Google Scholar]
  7. Donnenberg M. S., Kaper J. B. Enteropathogenic Escherichia coli. Infect Immun. 1992 Oct;60(10):3953–3961. doi: 10.1128/iai.60.10.3953-3961.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Donnenberg M. S., Yu J., Kaper J. B. A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J Bacteriol. 1993 Aug;175(15):4670–4680. doi: 10.1128/jb.175.15.4670-4680.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Drolet R., Fairbrother J. M., Harel J., Hélie P. Attaching and effacing and enterotoxigenic Escherichia coli associated with enteric colibacillosis in the dog. Can J Vet Res. 1994 Apr;58(2):87–92. [PMC free article] [PubMed] [Google Scholar]
  10. Drolet R., Fairbrother J. M., Vaillancourt D. Attaching and effacing Escherichia coli in a goat with diarrhea. Can Vet J. 1994 Feb;35(2):122–123. [PMC free article] [PubMed] [Google Scholar]
  11. Duhamel G. E., Moxley R. A., Maddox C. W., Erickson E. D. Enteric infection of a goat with enterohemorrhagic Escherichia coli (O103:H2). J Vet Diagn Invest. 1992 Apr;4(2):197–200. doi: 10.1177/104063879200400218. [DOI] [PubMed] [Google Scholar]
  12. Girón J. A., Ho A. S., Schoolnik G. K. An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science. 1991 Nov 1;254(5032):710–713. doi: 10.1126/science.1683004. [DOI] [PubMed] [Google Scholar]
  13. Gyles C. L. Escherichia coli cytotoxins and enterotoxins. Can J Microbiol. 1992 Jul;38(7):734–746. doi: 10.1139/m92-120. [DOI] [PubMed] [Google Scholar]
  14. Hacker J. Role of fimbrial adhesins in the pathogenesis of Escherichia coli infections. Can J Microbiol. 1992 Jul;38(7):720–727. doi: 10.1139/m92-118. [DOI] [PubMed] [Google Scholar]
  15. Harel J., Lapointe H., Fallara A., Lortie L. A., Bigras-Poulin M., Larivière S., Fairbrother J. M. Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea. J Clin Microbiol. 1991 Apr;29(4):745–752. doi: 10.1128/jcm.29.4.745-752.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Helie P., Morin M., Jacques M., Fairbrother J. M. Experimental infection of newborn pigs with an attaching and effacing Escherichia coli O45:K"E65" strain. Infect Immun. 1991 Mar;59(3):814–821. doi: 10.1128/iai.59.3.814-821.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jacques M., Foiry B. Electron microscopic visualization of capsular material of Pasteurella multocida types A and D labeled with polycationic ferritin. J Bacteriol. 1987 Aug;169(8):3470–3472. doi: 10.1128/jb.169.8.3470-3472.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Janke B. H., Francis D. H., Collins J. E., Libal M. C., Zeman D. H., Johnson D. D. Attaching and effacing Escherichia coli infections in calves, pigs, lambs, and dogs. J Vet Diagn Invest. 1989 Jan;1(1):6–11. doi: 10.1177/104063878900100104. [DOI] [PubMed] [Google Scholar]
  19. Jerse A. E., Gicquelais K. G., Kaper J. B. Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli. Infect Immun. 1991 Nov;59(11):3869–3875. doi: 10.1128/iai.59.11.3869-3875.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Jerse A. E., Kaper J. B. The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect Immun. 1991 Dec;59(12):4302–4309. doi: 10.1128/iai.59.12.4302-4309.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Knutton S., Baldini M. M., Kaper J. B., McNeish A. S. Role of plasmid-encoded adherence factors in adhesion of enteropathogenic Escherichia coli to HEp-2 cells. Infect Immun. 1987 Jan;55(1):78–85. doi: 10.1128/iai.55.1.78-85.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Knutton S., Lloyd D. R., McNeish A. S. Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infect Immun. 1987 Jan;55(1):69–77. doi: 10.1128/iai.55.1.69-77.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Law D. Adhesion and its role in the virulence of enteropathogenic Escherichia coli. Clin Microbiol Rev. 1994 Apr;7(2):152–173. doi: 10.1128/cmr.7.2.152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Levine M. M., Nataro J. P., Karch H., Baldini M. M., Kaper J. B., Black R. E., Clements M. L., O'Brien A. D. The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. J Infect Dis. 1985 Sep;152(3):550–559. doi: 10.1093/infdis/152.3.550. [DOI] [PubMed] [Google Scholar]
  25. Mathewson J. J., Cravioto A. HEp-2 cell adherence as an assay for virulence among diarrheagenic Escherichia coli. J Infect Dis. 1989 Jun;159(6):1057–1060. doi: 10.1093/infdis/159.6.1057. [DOI] [PubMed] [Google Scholar]
  26. Moon H. W., Whipp S. C., Argenzio R. A., Levine M. M., Giannella R. A. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect Immun. 1983 Sep;41(3):1340–1351. doi: 10.1128/iai.41.3.1340-1351.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Moxley R. A., Francis D. H. Natural and experimental infection with an attaching and effacing strain of Escherichia coli in calves. Infect Immun. 1986 Aug;53(2):339–346. doi: 10.1128/iai.53.2.339-346.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Nietfeld J. C., Tyler D. E., Harrison L. R., Cole J. R., Latimer K. S., Crowell W. A. Culture and morphologic features of small intestinal mucosal explants from weaned pigs. Am J Vet Res. 1991 Jul;52(7):1142–1146. [PubMed] [Google Scholar]
  29. Robins-Browne R. M., Tokhi A. M., Adams L. M., Bennett-Wood V., Moisidis A. V., Krejany E. O., O'Gorman L. E. Adherence characteristics of attaching and effacing strains of Escherichia coli from rabbits. Infect Immun. 1994 May;62(5):1584–1592. doi: 10.1128/iai.62.5.1584-1592.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sherman P. M., Soni R. Adherence of Vero cytotoxin-producing Escherichia coli of serotype O157:H7 to human epithelial cells in tissue culture: role of outer membranes as bacterial adhesins. J Med Microbiol. 1988 May;26(1):11–17. doi: 10.1099/00222615-26-1-11. [DOI] [PubMed] [Google Scholar]
  31. Tzipori S., Gibson R., Montanaro J. Nature and distribution of mucosal lesions associated with enteropathogenic and enterohemorrhagic Escherichia coli in piglets and the role of plasmid-mediated factors. Infect Immun. 1989 Apr;57(4):1142–1150. doi: 10.1128/iai.57.4.1142-1150.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tzipori S., Robins-Browne R. M., Gonis G., Hayes J., Withers M., McCartney E. Enteropathogenic Escherichia coli enteritis: evaluation of the gnotobiotic piglet as a model of human infection. Gut. 1985 Jun;26(6):570–578. doi: 10.1136/gut.26.6.570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wolf M. K., Andrews G. P., Fritz D. L., Sjogren R. W., Jr, Boedeker E. C. Characterization of the plasmid from Escherichia coli RDEC-1 that mediates expression of adhesin AF/R1 and evidence that AF/R1 pili promote but are not essential for enteropathogenic disease. Infect Immun. 1988 Aug;56(8):1846–1857. doi: 10.1128/iai.56.8.1846-1857.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Zhu C., Harel J., Jacques M., Desautels C., Donnenberg M. S., Beaudry M., Fairbrother J. M. Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea. Infect Immun. 1994 Oct;62(10):4153–4159. doi: 10.1128/iai.62.10.4153-4159.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES