Skip to main content
PMC home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1995 Apr;59(2):142–148.

Evaluation of an O antigen enzyme-linked immunosorbent assay for screening of milk samples for Salmonella dublin infection in dairy herds.

J Hoorfar 1, P Lind 1, V Bitsch 1
PMCID: PMC1263752  PMID: 7648527

Abstract

Levels of antibodies to the O antigens (O:1,9,12) of Salmonella dublin were tested in 1355 serum, 1143 cow milk and 160 bulk milk samples from dairy herds using an enzyme-linked immunosorbent assay (ELISA). In order to define the background reaction, milk samples from all lactating cows and serum samples from 9 animals were collected in each of 20 salmonellosis-free herds located on the island of Bornholm, where cattle salmonellosis has not been reported. Similar samples were collected from all stalled animals in 10 herds with recent (< 6 months) outbreaks of salmonellosis located in Jutland, where salmonella infection is enzootic. Using herd history of salmonellosis, herd location and clinical status of the herds as criteria, the optimal cutoff in the milk ELISA was determined as being at least 5% of the samples having optical density > 0.5, resulting in herd sensitivity of 1.0 and herd specificity of 0.95. While none of the sera in the herds from Bornholm was ELISA positive, 2 herds had a few reactors in the milk ELISA. Using the same cutoff, all but 1 bulk milk sample from 150 herds on Bornholm was ELISA-negative, and all 10 salmonellosis-positive herds from Jutland were ELISA-positive. A significant correlation was found between ELISA reactions in milk and in serum of cows (34% and 32% respectively, rs = 0.69, P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beh K. J. The origin of IgA-containing cells in intestinal lymph of sheep. Aust J Exp Biol Med Sci. 1977 Jun;55(3):263–274. doi: 10.1038/icb.1977.21. [DOI] [PubMed] [Google Scholar]
  2. Butler J. E. Bovine immunoglobulins: an augmented review. Vet Immunol Immunopathol. 1983 Mar;4(1-2):43–152. doi: 10.1016/0165-2427(83)90056-9. [DOI] [PubMed] [Google Scholar]
  3. Bán J., Zajac V., Altaner C., Cerný L. Early diagnosis of virus induced bovine leukosis in milk by a simple modified ELISA test. Zentralbl Veterinarmed B. 1982 Sep;29(8):591–595. doi: 10.1111/j.1439-0450.1982.tb01259.x. [DOI] [PubMed] [Google Scholar]
  4. Caffin J. P., Poutrel B., Rainard P. Physiological and pathological factors influencing bovine immunoglobulin G1 concentration in milk. J Dairy Sci. 1983 Oct;66(10):2161–2166. doi: 10.3168/jds.S0022-0302(83)82063-3. [DOI] [PubMed] [Google Scholar]
  5. Carlsson H. E., Lindberg A. A., Hammarström S. Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay. Infect Immun. 1972 Nov;6(5):703–708. doi: 10.1128/iai.6.5.703-708.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giles N., Hopper S. A., Wray C. Persistence of S. typhimurium in a large dairy herd. Epidemiol Infect. 1989 Oct;103(2):235–241. doi: 10.1017/s0950268800030582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoorfar J., Feld N. C., Schirmer A. L., Bitsch V., Lind P. Serodiagnosis of Salmonella dublin infection in Danish dairy herds using O-antigen based enzyme-linked immunosorbent assay. Can J Vet Res. 1994 Oct;58(4):268–274. [PMC free article] [PubMed] [Google Scholar]
  8. Jensen A. L., Poulsen J. S. Evaluation of diagnostic tests using relative operating characteristic (ROC) curves and the differential positive rate. An example using the total serum bile acid concentration and the alanine aminotransferase activity in the diagnosis of canine hepatobiliary diseases. Zentralbl Veterinarmed A. 1992 Nov;39(9):656–668. doi: 10.1111/j.1439-0442.1992.tb00231.x. [DOI] [PubMed] [Google Scholar]
  9. Lascelles A. K., Outteridge P. M., Mackenzie D. D. Local production of antibody by the lactating mammary gland following antigenic stimulation. Aust J Exp Biol Med Sci. 1966 Apr;44(2):169–180. doi: 10.1038/icb.1966.18. [DOI] [PubMed] [Google Scholar]
  10. McDowell G. H., Lascelles A. K. Local production of antibody by ovine mammary glands infused with Salmonella flagellar antigens. Aust J Exp Biol Med Sci. 1969 Dec;47(6):669–678. doi: 10.1038/icb.1969.164. [DOI] [PubMed] [Google Scholar]
  11. Newby T. J., Stokes C. R., Bourne F. J. Immunological activities of milk. Vet Immunol Immunopathol. 1982 Jan;3(1-2):67–94. doi: 10.1016/0165-2427(82)90032-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Niskanen R. Relationship between the levels of antibodies to bovine viral diarrhoea virus in bulk tank milk and the prevalence of cows exposed to the virus. Vet Rec. 1993 Oct 2;133(14):341–344. doi: 10.1136/vr.133.14.341. [DOI] [PubMed] [Google Scholar]
  13. Osborne A. D., Pearson H., Linton A. H., Shimeld C. Epidemiology of Salmonella infection in calves: the source of calfhood infection by Salmonella dublin. Vet Rec. 1977 Dec 24;101(26-27):513–516. [PubMed] [Google Scholar]
  14. Richardson A. Salmonellosis in cattle. Vet Rec. 1975 Apr 12;96(15):329–331. doi: 10.1136/vr.96.15.329. [DOI] [PubMed] [Google Scholar]
  15. Richardson A. The transmission of Salmonella dublin to calves from adult carrier cows. Vet Rec. 1973 Feb 3;92(5):112–115. doi: 10.1136/vr.92.5.112. [DOI] [PubMed] [Google Scholar]
  16. Richwald G. A., Greenland S., Johnson B. J., Friedland J. M., Goldstein E. J., Plichta D. T. Assessment of the excess risk of Salmonella dublin infection associated with the use of certified raw milk. Public Health Rep. 1988 Sep-Oct;103(5):489–493. [PMC free article] [PubMed] [Google Scholar]
  17. Robertsson J. A. Humoral antibody responses to experimental and spontaneous Salmonella infections in cattle measured by ELISA. Zentralbl Veterinarmed B. 1984 Jun;31(5):367–380. doi: 10.1111/j.1439-0450.1984.tb01314.x. [DOI] [PubMed] [Google Scholar]
  18. Smith B. P., Oliver D. G., Singh P., Dilling G., Martin P. A., Ram B. P., Jang L. S., Sharkov N., Orsborn J. S., Marvin P. A. Detection of Salmonella dublin mammary gland infection in carrier cows, using an enzyme-linked immunosorbent assay for antibody in milk or serum. Am J Vet Res. 1989 Aug;50(8):1352–1360. [PubMed] [Google Scholar]
  19. Watson D. L. Immunological functions of the mammary gland and its secretion--comparative review. Aust J Biol Sci. 1980 Aug;33(4):403–422. doi: 10.1071/bi9800403. [DOI] [PubMed] [Google Scholar]
  20. Werner S. B., Humphrey G. L., Kamei I. Association between raw milk and human Salmonella dublin infection. Br Med J. 1979 Jul 28;2(6184):238–241. doi: 10.1136/bmj.2.6184.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wray C., Wadsworth Q. C., Richards D. W., Morgan J. H. A three-year study of Salmonella dublin infection in a closed dairy herd. Vet Rec. 1989 May 20;124(20):532–537. doi: 10.1136/vr.124.20.532. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES