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. 1995 Jul;59(3):179–182.

Comparison of protection of experimentally challenged cattle vaccinated once or twice with a Pasteurella haemolytica bacterial extract vaccine.

J A Conlon 1, G F Gallo 1, P E Shewen 1, C Adlam 1
PMCID: PMC1263762  PMID: 8521349

Abstract

Three groups of calves (15-18 per group) were injected twice at a 3-week interval with 2 doses of phosphate buffered saline (PBS, CONTROL group), 2 doses of PRESPONSE, a Pasteurella haemolytica A1 bacterial extract vaccine (PRESPONSE-2 group) or 1 dose of PBS followed by a 2nd vaccination with 1 dose of PRESPONSE (PRESPONSE-1 group). Three weeks after the 2nd vaccination, the calves were challenged intratracheally with P. haemolytica A1. Calves were evaluated clinically for 3 days prior to challenge and for 5 days after challenge. Six days postchallenge, calves were either euthanized or sent to slaughter and the lungs were evaluated for percent pneumonic tissue. There was a significant effect of single or double application of vaccine on clinical scores (P = 0.0409). Percent pneumonic tissue at necropsy was significantly affected by vaccine group (P = 0.014). Calves in the CONTROL group had significantly higher percent pneumonic tissue after arcsine transformation (45.30%) than calves in any group receiving PRESPONSE, regardless of vaccination frequency (25.18% and 25.78%, for calves receiving 2 doses or 1 dose of PRESPONSE, respectively). Both serum toxin neutralizing and direct agglutinating titers were negatively correlated with percent pneumonic tissue. Most importantly, 1 dose of PRESPONSE was as efficient as 2 doses at eliciting a protective immune response. It is concluded that the presence of P. haemolytica as a natural commensal in the upper respiratory tract of the calf can effectively prime the animal, and allow the animal to respond in an anamnestic nature to only 1 dose of this vaccine.

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Selected References

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  1. Confer A. W., Lessley B. A., Panciera R. J., Fulton R. W., Kreps J. A. Serum antibodies to antigens derived from a saline extract of Pasteurella haemolytica: correlation with resistance to experimental bovine pneumonic pasteurellosis. Vet Immunol Immunopathol. 1985 Nov;10(2-3):265–278. doi: 10.1016/0165-2427(85)90052-2. [DOI] [PubMed] [Google Scholar]
  2. Confer A. W., Panciera R. J., Clinkenbeard K. D., Mosier D. A. Molecular aspects of virulence of Pasteurella haemolytica. Can J Vet Res. 1990 Apr;54 (Suppl):S48–S52. [PubMed] [Google Scholar]
  3. Confer A. W., Panciera R. J., Fulton R. W., Gentry M. J., Rummage J. A. Effect of vaccination with live or killed Pasteurella haemolytica on resistance to experimental bovine pneumonic pasteurellosis. Am J Vet Res. 1985 Feb;46(2):342–347. [PubMed] [Google Scholar]
  4. Confer A. W., Panciera R. J., Gentry M. J., Fulton R. W. Immunologic response to Pasteurella haemolytica and resistance against experimental bovine pneumonic pasteurellosis, induced by bacterins in oil adjuvants. Am J Vet Res. 1987 Feb;48(2):163–168. [PubMed] [Google Scholar]
  5. Conlon J. A., Shewen P. E. Clinical and serological evaluation of a Pasteurella haemolytica A1 capsular polysaccharide vaccine. Vaccine. 1993;11(7):767–772. doi: 10.1016/0264-410x(93)90263-w. [DOI] [PubMed] [Google Scholar]
  6. Conlon J. A., Shewen P. E., Lo R. Y. Efficacy of recombinant leukotoxin in protection against pneumonic challenge with live Pasteurella haemolytica A1. Infect Immun. 1991 Feb;59(2):587–591. doi: 10.1128/iai.59.2.587-591.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frank G. H., Briggs R. E., Gillette K. G. Pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. Am J Vet Res. 1987 Dec;48(12):1674–1677. [PubMed] [Google Scholar]
  8. Frank G. H., Smith P. C. Prevalence of Pasteurella haemolytica in transported calves. Am J Vet Res. 1983 Jun;44(6):981–985. [PubMed] [Google Scholar]
  9. Friend S. C., Thomson R. G., Wilkie B. N. Pulmonary lesions induced by Pasteurella hemolytica in cattle. Can J Comp Med. 1977 Apr;41(2):219–223. [PMC free article] [PubMed] [Google Scholar]
  10. Gentry M. J., Confer A. W., Panciera R. J. Serum neutralization of cytotoxin from Pasteurella haemolytica, serotype 1 and resistance to experimental bovine pneumonic pasteurellosis. Vet Immunol Immunopathol. 1985 Jul;9(3):239–250. doi: 10.1016/0165-2427(85)90074-1. [DOI] [PubMed] [Google Scholar]
  11. Greer C. N., Shewen P. E. Automated colorimetric assay for the detection of Pasteurella haemolytica leucotoxin. Vet Microbiol. 1986 Jun;12(1):33–42. doi: 10.1016/0378-1135(86)90039-8. [DOI] [PubMed] [Google Scholar]
  12. Jericho K. W., Langford E. V. Aerosol vaccination of calves with pasteurella haemolytica against experimental respiratory disease. Can J Comp Med. 1982 Jul;46(3):287–292. [PMC free article] [PubMed] [Google Scholar]
  13. Kelly A. P., Janzen E. D. A review of morbidity and mortality rates and disease occurrence in north american feedlot cattle. Can Vet J. 1986 Dec;27(12):496–500. [PMC free article] [PubMed] [Google Scholar]
  14. Purdy C. W., Livingston C. W., Jr, Frank G. H., Cummins J. M., Cole N. A., Loan R. W. A live Pasteurella haemolytica vaccine efficacy trial. J Am Vet Med Assoc. 1986 Mar 15;188(6):589–591. [PubMed] [Google Scholar]
  15. Shewen P. E., Wilkie B. N. Antibody titers to Pasteurella haemolytica A1 in Ontario beef cattle. Can J Comp Med. 1982 Oct;46(4):354–356. [PMC free article] [PubMed] [Google Scholar]
  16. Shewen P. E., Wilkie B. N. Evidence for the Pasteurella haemolytica cytotoxin as a product of actively growing bacteria. Am J Vet Res. 1985 May;46(5):1212–1214. [PubMed] [Google Scholar]
  17. Shewen P. E., Wilkie B. N. Vaccination of calves with leukotoxic culture supernatant from Pasteurella haemolytica. Can J Vet Res. 1988 Jan;52(1):30–36. [PMC free article] [PubMed] [Google Scholar]
  18. Wilkie B. N., Markham R. J., Shewen P. E. Response of calves to lung challenge exposure with Pasteurella haemolytica after parenteral or pulmonary immunization. Am J Vet Res. 1980 Nov;41(11):1773–1778. [PubMed] [Google Scholar]

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