Skip to main content
PMC home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1995 Oct;59(4):285–293.

Study of immune function in inbred miniature pigs vaccinated and challenged with suid herpesvirus 1.

P P Williams 1
PMCID: PMC1263783  PMID: 8548690

Abstract

Specific immune responses of inbred miniature pigs following vaccination and challenge with suid herpesvirus 1 (SHV-1) were determined. Vaccination of swine with SHV-1 elicited both specific neutralizing antibody and lymphoproliferative responses. Moreover, pigs vaccinated with SHV-1 were fully protected against a lethal virus challenge. Pigs vaccinated with a recombinant (r) SHV-1 virus, followed by challenge with a virulent SHV-1, had lower percentages of circulating T- and B-lymphocytes, and showed a significant (P < or = 0.05) reduction in peripheral blood mononuclear cell (PBMC) antibody-dependent cell-cytotoxicity than control (noninfected, SHV-1 sero-negative) animals. From the 5th through the 8th week of postchallenge, rSHV-1 was isolated from 2 of 4 pigs. Presence of r-virus was indicative that PBMC were infectious in vivo. The rSHV-1, with beta-galactosidase activity, was only recovered from ConA- and IL-2-stimulated primary PBMC cocultivated with porcine kidney cells. Control pigs exposed to challenge SHV-1 elicited both specific neutralizing antibody and lympho-proliferative responses followed by subsequent infection. These infected pigs, compared to control pigs, had significantly (P < or = 0.05) lowered percentages of T- and B-lymphocytes, lowered T-cell mitogenic responses, variable PBMC counts, and lowered blood phagocytic cell function. When PBMC from control pigs were cultured and infected with SHV-1, the virus caused a significant (P < or = 0.05) suppression of T-cell proliferation and PBMC mitochondrial dehydrogenase and macrophage activities.

Full text

PDF
285

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atluru D., Sayana U., Yang W. C., Schultz R. D. Inhibition of bovine lymphocyte response to phytohemagglutinin by a specific 5-lipoxygenase inhibitor. Vet Immunol Immunopathol. 1987 Mar;14(3):269–278. doi: 10.1016/0165-2427(87)90095-x. [DOI] [PubMed] [Google Scholar]
  2. Carmichael J., DeGraff W. G., Gazdar A. F., Minna J. D., Mitchell J. B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987 Feb 15;47(4):936–942. [PubMed] [Google Scholar]
  3. Ciobanu N., Welte K., Kruger G., Venuta S., Gold J., Feldman S. P., Wang C. Y., Koziner B., Moore M. A., Safai B. Defective T-cell response to PHA and mitogenic monoclonal antibodies in male homosexuals with acquired immunodeficiency syndrome and its in vitro correction by interleukin 2. J Clin Immunol. 1983 Oct;3(4):332–340. doi: 10.1007/BF00915794. [DOI] [PubMed] [Google Scholar]
  4. Farrar W. L., Anderson W. B. Interleukin-2 stimulates association of protein kinase C with plasma membrane. Nature. 1985 May 16;315(6016):233–235. doi: 10.1038/315233a0. [DOI] [PubMed] [Google Scholar]
  5. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. Isolation of a new herpesvirus from human CD4+ T cells. Proc Natl Acad Sci U S A. 1990 Jan;87(2):748–752. doi: 10.1073/pnas.87.2.748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gonzalez Juarrero M., Mebus C. A., Pan R., Revilla Y., Alonso J. M., Lunney J. K. Swine leukocyte antigen and macrophage marker expression on both African swine fever virus-infected and non-infected primary porcine macrophage cultures. Vet Immunol Immunopathol. 1992 May;32(3-4):243–259. doi: 10.1016/0165-2427(92)90049-V. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henderson L. M., Katz J. B., Erickson G. A., Mayfield J. E. In vivo and in vitro genetic recombination between conventional and gene-deleted vaccine strains of pseudorabies virus. Am J Vet Res. 1990 Oct;51(10):1656–1662. [PubMed] [Google Scholar]
  8. Iglesias G., Pijoan C., Molitor T. Interactions of Pseudorabies virus with swine alveolar macrophages I: virus replication. Arch Virol. 1989;104(1-2):107–115. doi: 10.1007/BF01313812. [DOI] [PubMed] [Google Scholar]
  9. Kawashima K., Platt K. B. The effect of human recombinant interleukin-2 on the porcine immune response to a pseudorabies virus subunit vaccine. Vet Immunol Immunopathol. 1989 Nov 15;22(4):345–353. doi: 10.1016/0165-2427(89)90170-0. [DOI] [PubMed] [Google Scholar]
  10. Kerman R., Smith R., Ezdinli E., Stefani S. Unification and technical aspects of total t, active t and b lymphocyte rosette assays. Immunol Commun. 1976;5(7-8):685–694. doi: 10.3109/08820137609033876. [DOI] [PubMed] [Google Scholar]
  11. Klobasa F., Butler J. E., Werhahn E., Habe F. Maternal-neonatal immunoregulation in swine. II. Influence of multiparity on de novo immunoglobulin synthesis by piglets. Vet Immunol Immunopathol. 1986 Feb;11(2):149–159. doi: 10.1016/0165-2427(86)90094-2. [DOI] [PubMed] [Google Scholar]
  12. Lowenthal J. W., Cerottini J. C., MacDonald H. R. Interleukin 1-dependent induction of both interleukin 2 secretion and interleukin 2 receptor expression by thymoma cells. J Immunol. 1986 Aug 15;137(4):1226–1231. [PubMed] [Google Scholar]
  13. Martins C. L., Leitão A. C. Porcine immune responses to African swine fever virus (ASFV) infection. Vet Immunol Immunopathol. 1994 Oct;43(1-3):99–106. doi: 10.1016/0165-2427(94)90125-2. [DOI] [PubMed] [Google Scholar]
  14. Mengeling W. L., Gutekunst D. E., Fernelius A. L., Pirtle E. C. Demonstration of an Antigenic Relationship Between Hog Cholera and Bovine Viral Diarrhea Viruses by Immunofluorescence. Can J Comp Med Vet Sci. 1963 Jul;27(7):162–164. [PMC free article] [PubMed] [Google Scholar]
  15. Muneer M. A., Farah I. O., Newman J. A., Goyal S. M. Immunosuppression in animals. Br Vet J. 1988 May-Jun;144(3):288–301. doi: 10.1016/0007-1935(88)90116-9. [DOI] [PubMed] [Google Scholar]
  16. Narita M., Imada T., Takada M., Ishibashi K., Nemoto F., Haritani M., Kobayashi M. Detection of reactivating pseudorabies virus in tissue by immunoperoxidase technique. J Comp Pathol. 1989 Aug;101(2):151–159. doi: 10.1016/0021-9975(89)90063-7. [DOI] [PubMed] [Google Scholar]
  17. Onyekaba C., Bueon L., King P., Fahrmann J., Goyal S. M. Susceptibility of various cell culture systems to pseudorabies virus. Comp Immunol Microbiol Infect Dis. 1987;10(3-4):163–166. doi: 10.1016/0147-9571(87)90027-0. [DOI] [PubMed] [Google Scholar]
  18. Page G. R., Wang F. I., Hahn E. C. Interaction of pseudorabies virus with porcine peripheral blood lymphocytes. J Leukoc Biol. 1992 Oct;52(4):441–448. doi: 10.1002/jlb.52.4.441. [DOI] [PubMed] [Google Scholar]
  19. Rock D. L., Hagemoser W. A., Osorio F. A., McAllister H. A. Transcription from the pseudorabies virus genome during latent infection. Brief report. Arch Virol. 1988;98(1-2):99–106. doi: 10.1007/BF01321010. [DOI] [PubMed] [Google Scholar]
  20. Rosoff P. M., Savage N., Dinarello C. A. Interleukin-1 stimulates diacylglycerol production in T lymphocytes by a novel mechanism. Cell. 1988 Jul 1;54(1):73–81. doi: 10.1016/0092-8674(88)90181-x. [DOI] [PubMed] [Google Scholar]
  21. Rouse B. T., Horohov D. W. Immunosuppression in viral infections. Rev Infect Dis. 1986 Nov-Dec;8(6):850–873. doi: 10.1093/clinids/8.6.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rziha H. J., Mettenleiter T. C., Ohlinger V., Wittmann G. Herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral DNA in neural tissues. Virology. 1986 Dec;155(2):600–613. doi: 10.1016/0042-6822(86)90220-5. [DOI] [PubMed] [Google Scholar]
  23. Sachs D. H., Leight G., Cone J., Schwarz S., Stuart L., Rosenberg S. Transplantation in miniature swine. I. Fixation of the major histocompatibility complex. Transplantation. 1976 Dec;22(6):559–567. doi: 10.1097/00007890-197612000-00004. [DOI] [PubMed] [Google Scholar]
  24. Smith K. A. Interleukin-2: inception, impact, and implications. Science. 1988 May 27;240(4856):1169–1176. doi: 10.1126/science.3131876. [DOI] [PubMed] [Google Scholar]
  25. Suganuma A., Ishizuka A., Sakiyama Y., Maede Y., Namioka S. B lymphocyte differentiation and suppressor activity by T lymphocytes derived from neonatal and sucking piglets. Res Vet Sci. 1986 May;40(3):400–405. [PubMed] [Google Scholar]
  26. Thoren-Tolling K. Chemiluminescence and chemotaxis assay of porcine polymorphonuclear cells. A methodological study. Zentralbl Veterinarmed A. 1990 Apr;37(3):174–185. doi: 10.1111/j.1439-0442.1990.tb00892.x. [DOI] [PubMed] [Google Scholar]
  27. Williams J. M., Deloria D., Hansen J. A., Dinarello C. A., Loertscher R., Shapiro H. M., Strom T. B. The events of primary T cell activation can be staged by use of Sepharose-bound anti-T3 (64.1) monoclonal antibody and purified interleukin 1. J Immunol. 1985 Oct;135(4):2249–2255. [PubMed] [Google Scholar]
  28. Williams P. P., Hall M. R., McFarland M. D. Immunological responses of cross-bred and in-bred miniature pigs to swine poxvirus. Vet Immunol Immunopathol. 1989 Nov 30;23(1-2):149–159. doi: 10.1016/0165-2427(89)90117-7. [DOI] [PubMed] [Google Scholar]
  29. Williams P. P. Immunomodulating effects of intestinal absorbed maternal colostral leukocytes by neonatal pigs. Can J Vet Res. 1993 Jan;57(1):1–8. [PMC free article] [PubMed] [Google Scholar]
  30. Zsak L., Mettenleiter T. C., Sugg N., Ben-Porat T. Release of pseudorabies virus from infected cells is controlled by several viral functions and is modulated by cellular components. J Virol. 1989 Dec;63(12):5475–5477. doi: 10.1128/jvi.63.12.5475-5477.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES