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. 1996 Jul;60(3):161–171.

The relationship between respiratory exchange ratio, plasma lactate and muscle lactate concentrations in exercising horses using a valved gas collection system.

G M Gauvreau 1, S S Young 1, H Staempfli 1, L J McCutcheon 1, B A Wilson 1, W N McDonell 1
PMCID: PMC1263828  PMID: 8809378

Abstract

A valved gas collection system for horses was validated, then used to examine the relationship between the respiratory exchange ratio (RER), and plasma and muscle lactate in exercising horses. Four healthy Standardbred horses were trained to breathe through the apparatus while exercising on a treadmill. Comparisons of arterial blood gas tensions were made at 3 work levels for each horse, without (control), and with the gas collection system present. At the highest work level, the arterial oxygen tension (PaO2) was significantly lower (P < 0.05), and the arterial carbon dioxide tension (PaCO2) was significantly higher (P < 0.05), than control levels when the apparatus was present; however arterial oxygen content remained unchanged. The horses completed a standardized incremental treadmill test on 4 occasions to determine the repeatability of measurements of oxygen consumption (VO2), carbon dioxide production (VCO2), inspired minute ventilation (VI), respiratory exchange ratio (RER), ventilatory equivalent for oxygen (VI/VO2), tidal volume (VT), and ventilatory frequency (VF). All gas exchange and respiratory measurements showed good reproducibility with the mean coefficient of variation of the 4 horses ranging from 3.8 to 12%. We examined the relationship between 3 indices of energy metabolism in horses performing treadmill exercise: respiratory exchange ratio (RER), central venous plasma and muscle lactate concentrations. A relationship between RER and plasma lactate concentration was established. To compare muscle and plasma lactate concentrations, the horses completed a discontinuous exercise test without the gas collection apparatus present. Significant relationships (P < 0.05), between plasma lactate concentration and RER, and between plasma and muscle lactate concentration, were described for each horse. The valved gas collection system produced a measurable but tolerable degree of interference to respiration, and provided reproducible measurements of gas exchange and ventilatory measurements. It was concluded that measurements of both gas exchange and blood lactate may be used to indicate increased glycolytic activity within exercising skeletal muscle.

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Selected References

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  1. Art T., Lekeux P. Training-induced modifications in cardiorespiratory and ventilatory measurements in thoroughbred horses. Equine Vet J. 1993 Nov;25(6):532–536. doi: 10.1111/j.2042-3306.1993.tb03008.x. [DOI] [PubMed] [Google Scholar]
  2. Bayly W. M., Gabel A. A., Barr S. A. Cardiovascular effects of submaximal aerobic training on a treadmill in Standardbred horses, using a standardized exercise test. Am J Vet Res. 1983 Apr;44(4):544–553. [PubMed] [Google Scholar]
  3. Bayly W. M., Hodgson D. R., Schulz D. A., Dempsey J. A., Gollnick P. D. Exercise-induced hypercapnia in the horse. J Appl Physiol (1985) 1989 Nov;67(5):1958–1966. doi: 10.1152/jappl.1989.67.5.1958. [DOI] [PubMed] [Google Scholar]
  4. Bayly W. M., Schultz D. A., Hodgson D. R., Gollnick P. D. Ventilatory responses of the horse to exercise: effect of gas collection systems. J Appl Physiol (1985) 1987 Sep;63(3):1210–1217. doi: 10.1152/jappl.1987.63.3.1210. [DOI] [PubMed] [Google Scholar]
  5. Chwalbinska-Moneta J., Robergs R. A., Costill D. L., Fink W. J. Threshold for muscle lactate accumulation during progressive exercise. J Appl Physiol (1985) 1989 Jun;66(6):2710–2716. doi: 10.1152/jappl.1989.66.6.2710. [DOI] [PubMed] [Google Scholar]
  6. Evans D. L., Rose R. J. Cardiovascular and respiratory responses in Thoroughbred horses during treadmill exercise. J Exp Biol. 1988 Jan;134:397–408. doi: 10.1242/jeb.134.1.397. [DOI] [PubMed] [Google Scholar]
  7. Evans D. L., Rose R. J. Determination and repeatability of maximum oxygen uptake and other cardiorespiratory measurements in the exercising horse. Equine Vet J. 1988 Mar;20(2):94–98. doi: 10.1111/j.2042-3306.1988.tb01467.x. [DOI] [PubMed] [Google Scholar]
  8. Evans D. L., Rose R. J. Dynamics of cardiorespiratory function in Standardbred horses during different intensities of constant-load exercise. J Comp Physiol B. 1988;157(6):791–799. doi: 10.1007/BF00691010. [DOI] [PubMed] [Google Scholar]
  9. Evans D. L., Rose R. J. Effect of a respiratory gas collection mask on some measurements of cardiovascular and respiratory function in horses exercising on a treadmill. Res Vet Sci. 1988 Mar;44(2):220–225. [PubMed] [Google Scholar]
  10. Gallivan G. J., Bignell W., McDonell W. N., Whiting T. L. Simple nonrebreathing valves for use with large mammals. Can J Vet Res. 1989 Apr;53(2):143–146. [PMC free article] [PubMed] [Google Scholar]
  11. Gallivan G. J., Viel L., McDonell W. N. An evaluation of the multiple-breath nitrogen washout as a pulmonary function test in horses. Can J Vet Res. 1990 Jan;54(1):99–105. [PMC free article] [PubMed] [Google Scholar]
  12. Gauvreau G. M., Staempfli H., McCutcheon L. J., Young S. S., McDonell W. N. Comparison of aerobic capacity between racing standardbred horses. J Appl Physiol (1985) 1995 Apr;78(4):1447–1451. doi: 10.1152/jappl.1995.78.4.1447. [DOI] [PubMed] [Google Scholar]
  13. Gollnick P. D., Saltin B. Significance of skeletal muscle oxidative enzyme enhancement with endurance training. Clin Physiol. 1982 Feb;2(1):1–12. doi: 10.1111/j.1475-097x.1982.tb00001.x. [DOI] [PubMed] [Google Scholar]
  14. Hodgson D. R., McCutcheon L. J., Byrd S. K., Brown W. S., Bayly W. M., Brengelmann G. L., Gollnick P. D. Dissipation of metabolic heat in the horse during exercise. J Appl Physiol (1985) 1993 Mar;74(3):1161–1170. doi: 10.1152/jappl.1993.74.3.1161. [DOI] [PubMed] [Google Scholar]
  15. Jones J. H., Longworth K. E., Lindholm A., Conley K. E., Karas R. H., Kayar S. R., Taylor C. R. Oxygen transport during exercise in large mammals. I. Adaptive variation in oxygen demand. J Appl Physiol (1985) 1989 Aug;67(2):862–870. doi: 10.1152/jappl.1989.67.2.862. [DOI] [PubMed] [Google Scholar]
  16. Jones J. H., Taylor C. R., Lindholm A., Straub R., Longworth K. E., Karas R. H. Blood gas measurements during exercise: errors due to temperature correction. J Appl Physiol (1985) 1989 Aug;67(2):879–884. doi: 10.1152/jappl.1989.67.2.879. [DOI] [PubMed] [Google Scholar]
  17. Lindholm A., Piehl K. Fibre composition, enzyme activity and concentrations of metabolites and electrolytes in muscles of standardbred horses. Acta Vet Scand. 1974;15(3):287–309. doi: 10.1186/BF03547460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Milne D. W., Skarda R. T., Gabel A. A., Smith L. G., Ault K. Effects of training on biochemical values in standardbred horses. Am J Vet Res. 1976 Mar;37(3):285–290. [PubMed] [Google Scholar]
  19. Morris E. A., Seeherman H. J. Clinical evaluation of poor performance in the racehorse: the results of 275 evaluations. Equine Vet J. 1991 May;23(3):169–174. doi: 10.1111/j.2042-3306.1991.tb02749.x. [DOI] [PubMed] [Google Scholar]
  20. Rose R. J., Hodgson D. R., Kelso T. B., McCutcheon L. J., Reid T. A., Bayly W. M., Gollnick P. D. Maximum O2 uptake, O2 debt and deficit, and muscle metabolites in Thoroughbred horses. J Appl Physiol (1985) 1988 Feb;64(2):781–788. doi: 10.1152/jappl.1988.64.2.781. [DOI] [PubMed] [Google Scholar]
  21. Seeherman H. J., Morris E. A. Application of a standardised treadmill exercise test for clinical evaluation of fitness in 10 thoroughbred racehorses. Equine Vet J Suppl. 1990 Jun;(9):26–34. doi: 10.1111/j.2042-3306.1990.tb04730.x. [DOI] [PubMed] [Google Scholar]
  22. Seeherman H. J., Morris E. A. Methodology and repeatability of a standardised treadmill exercise test for clinical evaluation of fitness in horses. Equine Vet J Suppl. 1990 Jun;(9):20–25. doi: 10.1111/j.2042-3306.1990.tb04729.x. [DOI] [PubMed] [Google Scholar]
  23. Thomas D. P., Fregin G. F., Gerber N. H., Ailes N. B. Effects of training on cardiorespiratory function in the horse. Am J Physiol. 1983 Aug;245(2):R160–R165. doi: 10.1152/ajpregu.1983.245.2.R160. [DOI] [PubMed] [Google Scholar]
  24. Varène P., Vieillefond H., Saumon G., Lafosse J. E. Etalonnage des pneumotachographes par méthode intégrale. Bull Physiopathol Respir (Nancy) 1974 May-Jun;10(3):349–360. [PubMed] [Google Scholar]
  25. Wasserman K., Beaver W. L., Whipp B. J. Mechanisms and patterns of blood lactate increase during exercise in man. Med Sci Sports Exerc. 1986 Jun;18(3):344–352. doi: 10.1249/00005768-198606000-00017. [DOI] [PubMed] [Google Scholar]

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