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. 1965 Nov;97(2):573–578. doi: 10.1042/bj0970573

Production and purification of bacilysin

Henry J Rogers 1,*, G G F Newton 1, E P Abraham 1
PMCID: PMC1264678  PMID: 16749167

Abstract

1. Bacilysin, a hydrophilic substance formed by certain aerobic spore-forming bacteria that causes lysis in cultures of growing staphylococci, has been produced in aerated cultures of a strain of Bacillus subtilis (A14). A chemically defined medium was used, which contained glucose, Czapek–Dox salts and ferric iron. Production of bacilysin occurred, after a lag, while the culture was still undergoing rapid growth. 2. Bacilysin was adsorbed from the culture medium on Zeo-Karb 225 (SR5) (H+ form) and eluted with aqueous pyridine. The crude material was purified by chromatography in pyridine–acetate buffers on columns of Dowex 50 (X2) and Dowex 50 (X8) respectively and by chromatography in aq. 70% (v/v) propan-2-ol on Sephadex G-25. 3. Purified bacilysin behaved as a single ninhydrin-positive substance when subjected to chromatography on paper in butan-1-ol–acetic acid–water and to electrophoresis on paper at pH4·5 or pH1·8. At pH4·5 the substance behaved as though it had no net change and at pH1·8 it migrated towards the cathode.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Foster J. W., Woodruff H. B. Bacillin, a New Antibiotic Substance from a Soil Isolate of Bacillus subtilis. J Bacteriol. 1946 Mar;51(3):363–369. [PMC free article] [PubMed] [Google Scholar]
  2. Heatley N. G. A method for the assay of penicillin. Biochem J. 1944;38(1):61–65. doi: 10.1042/bj0380061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. JEPSON J. B., SMITH I. Multiple dipping procedures in paper chromatography: a specific test for hydroxy-proline. Nature. 1953 Dec 12;172(4389):1100–1101. doi: 10.1038/1721100b0. [DOI] [PubMed] [Google Scholar]
  4. KATZ A. M., DREYER W. J., ANFINSEN C. B. Peptide separation by two-dimensional chromatography and electrophoresis. J Biol Chem. 1959 Nov;234:2897–2900. [PubMed] [Google Scholar]
  5. KAY D., FILDES P. The calcium requirement of a typhoid bacteriophage. Br J Exp Pathol. 1950 Jun;31(3):338–348. [PMC free article] [PubMed] [Google Scholar]
  6. Koffler H., Knight S. G., Frazier W. C. The Effect of Certain Mineral Elements on the Production of Penicillin in Shake Flasks. J Bacteriol. 1947 Jan;53(1):115–123. doi: 10.1128/jb.53.1.115-123.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MOORE S., STEIN W. H. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J Biol Chem. 1954 Dec;211(2):907–913. [PubMed] [Google Scholar]
  8. NEWTON G. G. F. Antibiotics from a strain of B. subtilis; bacilipin A and B and bacilysin. Br J Exp Pathol. 1949 Aug;30(4):306-19, pl. [PMC free article] [PubMed] [Google Scholar]
  9. Rogers H. J., Lomakina N., Abraham E. P. Observations on the structure of bacilysin. Biochem J. 1965 Nov;97(2):579–586. doi: 10.1042/bj0970579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Woodruff H. B., Foster J. W. Antibacillin, a Naturally Occurring Inhibitor of Bacillin. J Bacteriol. 1946 Mar;51(3):371–380. [PMC free article] [PubMed] [Google Scholar]

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