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. 1965 Nov;97(2):587–594. doi: 10.1042/bj0970587

Steady-state concentrations of coenzyme A, acetyl-coenzyme A and long-chain fatty acyl-coenzyme A in rat-liver mitochondria oxidizing palmitate

P B Garland 1, D Shepherd 1, D W Yates 1
PMCID: PMC1264680  PMID: 16749169

Abstract

1. Fluorimetric assays are described for CoASH, acetyl-CoA and long-chain fatty acyl-CoA, and are sensitive to at least 50μμmoles of each. 2. Application of these assays to rat-liver mitochondria oxidizing palmitate in the absence and presence of carnitine indicated two pools of intramitochondrial CoA. One pool could be acylated by palmitate and ATP, and the other pool acylated by palmitate with ATP and carnitine, or by palmitoylcarnitine alone. 3. The intramitochondrial content of acetyl-CoA is increased by the oxidation of palmitate both in the absence and presence of l-malate. 4. The conversion of palmitoyl-CoA into acetyl-CoA by β-oxidation takes place without detectable accumulation of acyl-CoA intermediates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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