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. 1966 Jan;98(1):38–43. doi: 10.1042/bj0980038

Metabolism of iodomethane in the rat

M K Johnson 1
PMCID: PMC1264791  PMID: 5938661

Abstract

1. The LD50 of iodomethane orally administered to rats is 76mg./kg. body wt. but repeated daily doses of 30–50mg./kg. body wt. are without effect. 2. Oral doses of iodomethane to rats are rapidly converted into S-methylglutathione in the liver, and S-methylglutathione is excreted in the bile. 3. The conjugation process has been reproduced in vitro and shown to be enzymically catalysed. 4. S-Methylglutathione is degraded to S-methylcysteine by kidney homogenates. 5. Only a small proportion of a dose of iodomethane or of S-methylcysteine is excreted as compounds related to methylmercapturic acid. 6. Paper chromatography of extracts of livers from normal and dosed rats supports the view that GSH is the predominant non-protein thiol present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON E. I., MOSHER W. A. Incorporation of S35 from DL-cystine into glutathione and protein in the rat. J Biol Chem. 1951 Feb;188(2):717–722. [PubMed] [Google Scholar]
  2. BARNES M. M., JAMES S. P., WOOD P. B. The formation of mercapturic acids. 1. Formation of mercapturic acid and the levels of glutathione in tissues. Biochem J. 1959 Apr;71(4):680–690. doi: 10.1042/bj0710680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BARNSLEY E. A. THE METABOLISM OF S-METHYL-L-CYSTEINE IN THE RAT. Biochim Biophys Acta. 1964 Jul 15;90:24–36. doi: 10.1016/0304-4165(64)90115-1. [DOI] [PubMed] [Google Scholar]
  4. BARNSLEY E. A., YOUNG L. BIOCHEMICAL STUDIES OF TOXIC AGENTS. THE METABOLISM OF IODOMETHANE. Biochem J. 1965 Apr;95:77–81. doi: 10.1042/bj0950077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BEUTLER E., DURON O., KELLY B. M. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963 May;61:882–888. [PubMed] [Google Scholar]
  6. BINKLEY F. Enzymatic cleavage of thioethers. J Biol Chem. 1950 Sep;186(1):287–296. [PubMed] [Google Scholar]
  7. Barnsley E. A., Thomson A. E., Young L. Biochemical studies of toxic agents. 15. The biosynthesis of ethylmercapturic acid sulphoxide. Biochem J. 1964 Mar;90(3):588–596. doi: 10.1042/bj0900588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bray H. G., Caygill J. C., James S. P., Wood P. B. Formation of mercapturic acids. 5. Metabolism of some halogenoparaffins and nitroparaffins. Biochem J. 1964 Jan;90(1):127–132. doi: 10.1042/bj0900127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. CALCUTT G., DOXEY D. The apparent glutathione content of some normal tissues and some animal tumours. Br J Cancer. 1962 Sep;16:562–569. doi: 10.1038/bjc.1962.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Davidson B. E., Hird F. J. The estimation of glutathione in rat tissues. A comparison of a new spectrophotometric method with the glyoxalase method. Biochem J. 1964 Nov;93(2):232–236. doi: 10.1042/bj0930232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dent C. E. A study of the behaviour of some sixty amino-acids and other ninhydrin-reacting substances on phenol-;collidine' filter-paper chromatograms, with notes as to the occurrence of some of them in biological fluids. Biochem J. 1948;43(2):169–180. [PMC free article] [PubMed] [Google Scholar]
  12. GAITONDE M. K. The composition of brain lipoprotein fractions obtained by different procedures. J Neurochem. 1961 Dec;8:234–242. doi: 10.1111/j.1471-4159.1961.tb13548.x. [DOI] [PubMed] [Google Scholar]
  13. GRENBY T. H., YOUNG L. Biochemical studies on toxic agents. 12. The biosynthesis of n-propylmercapturic acid from n-propyl halides. Biochem J. 1960 Apr;75:28–33. doi: 10.1042/bj0750028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. GRODSKY G. M., CARBONE J. V., FANSKA R. Identification of metabolites of sulfobromophthalein. J Clin Invest. 1959 Nov;38:1981–1988. doi: 10.1172/JCI103977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. GRUNERT R. R., PHILLIPS P. H. A modification of the nitroprusside method of analysis for glutathione. Arch Biochem. 1951 Feb;30(2):217–225. [PubMed] [Google Scholar]
  16. HORNER W. H., KUCHINSKAS E. J. Metabolism of methyllabeled S-methylcysteine in the rat. J Biol Chem. 1959 Nov;234:2935–2937. [PubMed] [Google Scholar]
  17. Johnson M. K. Studies on glutathione S-alkyltransferase of the rat. Biochem J. 1966 Jan;98(1):44–56. doi: 10.1042/bj0980044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Johnson M. K. The influence of some aliphatic compounds on rat liver glutathione levels. Biochem Pharmacol. 1965 Sep;14(9):1383–1385. doi: 10.1016/0006-2952(65)90122-x. [DOI] [PubMed] [Google Scholar]
  19. KALSER S. C., BECK L. V. Turnover of liver glutathione in trauma. Biochem J. 1963 Jun;87:618–623. doi: 10.1042/bj0870618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. KNIGHT R. H., YOUNG L. Biochemical studies of toxic agents. 11. The occurrence of premercapturic acids. Biochem J. 1958 Sep;70(1):111–119. doi: 10.1042/bj0700111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. MAW G. A. Observations on the late of some aliphatic sulphonic acids in the rat. Biochem J. 1953 Aug;55(1):37–41. doi: 10.1042/bj0550037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. MAZEL P., HENDERSON J. F., AXELROD J. S-DEMETHYLATION BY MICROSOMAL ENZYMES. J Pharmacol Exp Ther. 1964 Jan;143:1–6. [PubMed] [Google Scholar]
  23. Markham R. Improvements to the ultraviolet-absorption optical system of an ultracentrifuge. Biochem J. 1963 Apr;87(1):9–11. doi: 10.1042/bj0870009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. OWENS C. W., BELCHER R. V. A COLORIMETRIC MICRO-METHOD FOR THE DETERMINATION OF GLUTATHIONE. Biochem J. 1965 Mar;94:705–711. doi: 10.1042/bj0940705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. THOMSON A. E., BARNSLEY E. A., YOUNG L. Biochemical studies of toxic agents. 14. The biosynthesis of ethylmercapturic acid. Biochem J. 1963 Jan;86:145–152. doi: 10.1042/bj0860145. [DOI] [PMC free article] [PubMed] [Google Scholar]

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