Abstract
1. The nature of the precursors of the xylene ring in riboflavine was reinvestigated with growing as well as resting cells of Eremothecium ashbyii. 2. The incorporation of acetoin into riboflavine was very low; further, [2-14C]pyruvate and [1-14C]acetate were equally effective as precursors of lumichrome, and pyruvate was much more active as a precursor of acetoin. These results exclude acetoin as a direct precursor of riboflavine. 3. Addition of unlabelled glucose decreased the incorporation of [14C]acetate into riboflavine more than it decreased the conversion of acetate into carbon dioxide, indicating that acetate is not a direct riboflavine precursor. 4. The incorporation of various sugars and dilution experiments suggest that a derivative of the intermediates of the pentose phosphate cycle is the precursor of the xylene ring in riboflavine.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BRADY R. O., RABINOWITZ J., VAN BAALEN J., GURIN S. The synthesis of radioactive cholesterol and fatty acids in vitro. II. A further study of precursors. J Biol Chem. 1951 Nov;193(1):137–143. [PubMed] [Google Scholar]
- GOODWIN T. W., JONES O. T. Studies on the biosynthesis of riboflavin. 3. The utilization of 14C-labelled serine for riboflavin biosynthesis by Eremothecium ashbyii. Biochem J. 1956 Sep;64(1):9–13. doi: 10.1042/bj0640009. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MASUDA T. Application of chromatography. XXIX. G compound isolated from the mycelium of Eremothecium ashbyii. Pharm Bull. 1956 Oct;4(5):375–381. doi: 10.1248/cpb1953.4.375. [DOI] [PubMed] [Google Scholar]
- McNUTT W. S. The direct contribution of adenine to the biogenesis of riboflavin by Eremothecium ashbyii. J Biol Chem. 1954 Oct;210(2):511–519. [PubMed] [Google Scholar]
- PLAUT G. W., BROBERG P. L. Biosynthesis of riboflavin. III. Incorporation of C14-labeled compounds into the ribityl side chain. J Biol Chem. 1956 Mar;219(1):131–138. [PubMed] [Google Scholar]
- PLAUT G. W. Biosynthesis of riboflavin. II. Incorporation of C14-labeled compounds into ring A. J Biol Chem. 1954 Nov;211(1):111–116. [PubMed] [Google Scholar]
- WEIL-MALHERBE H., BONE A. D. The association of adrenaline and noradrenaline with blood platelets. Biochem J. 1958 Sep;70(1):14–22. doi: 10.1042/bj0700014. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHITBY L. G. Riboflavinyl glucoside; a new derivative of riboflavin. Biochem J. 1952 Feb;50(4):433–438. doi: 10.1042/bj0500433. [DOI] [PMC free article] [PubMed] [Google Scholar]