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. 1966 Jan;98(1):347–352. doi: 10.1042/bj0980347

Purification and properties of galactokinase from pig liver

F J Ballard 1
PMCID: PMC1264838  PMID: 5938660

Abstract

1. Galactokinase has been purified from the liver of young pigs by high-speed centrifugation, chromatography on Sephadex G-100 and DEAE-cellulose, and ammonium sulphate fractionation. 2. The enzyme preparation has a specific activity of 10–18μmoles of galactose phosphorylated/mg. of protein/min. at 37° and has been purified 400-fold from the liver supernatant. 3. Purified liver galactokinase has Michaelis constants of 1×10−4−3×10−4m for galactose and 2×10−4m for ATP–Mg2+, and the enzyme reaction produces equimolar amounts of galactose 1-phosphate and ADP. 4. Galactokinase phosphorylates 2-deoxygalactose and galactosamine in addition to galactose, has a pH optimum of 7·8, a Q10 of 2, and is stimulated by cysteine and other thiols. 5. With the exception of substrate specificity, the properties of liver galactokinase are similar to galactokinase purified from yeast and Escherichia coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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