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. 1966 Feb;98(2):405–409. doi: 10.1042/bj0980405

Purification of β-acetylglucosaminase and β-galactosidase from ram testis

J C Caygill 1,*, Christine P J Roston 1, F R Jevons 1
PMCID: PMC1264859  PMID: 5949569

Abstract

1. The presence of β-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of β-acetylglucosaminase (EC 3.2.1.30) and of β-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the β-acetylglucosaminase 35 times and for the β-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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