Abstract
1. The effect of various changes in the composition of the supporting medium on the capacity of isolated rat diaphragm to incorporate amino acids into its protein has been studied. 2. Replacement of most of the normal ionic constituents by sucrose is inhibitory towards protein synthesis, as is also substitution of choline or K+ for Na+. 3. The capacity of the tissue to respond to a stimulatory effect of insulin is impaired in the sucrose media and under certain conditions in the absence of Na+, particularly when Na+ is replaced by K+ and the 14C-labelled amino acid is presented at a relatively high concentration. 4. Cutting of the tissue before incubation also decreases incorporating capacity and markedly decreases responsiveness to insulin. 5. In abnormal media the cellular content of ATP falls sharply. 6. The ATP content of the tissue also declines in the presence of 2-deoxyglucose. This change is prevented by the addition of glucose but not of pyruvate and succinate. 7. Although affecting the rate of amino acid incorporation the ATP content is not thought generally to limit sensitivity to insulin.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BHATTACHARYA G. Influence of ions on the uptake of glucose and on the effect of insulin on it by rat diaphragm. Nature. 1959 Jan 31;183(4657):324–325. doi: 10.1038/183324a0. [DOI] [PubMed] [Google Scholar]
- BHATTACHARYA G. Role of magnesium in the insulin-sensitivity of the rat diaphragm in vitro. Nature. 1959 Oct 31;184(Suppl 18):1401–1402. doi: 10.1038/1841401b0. [DOI] [PubMed] [Google Scholar]
- BORSOOK H., DEASY C. L., HAAGENSMIT A. J., KEIGHLEY G., LOWY P. H. Incorporation in vitro of labeled amino acids into rat diaphragm proteins. J Biol Chem. 1950 Sep;186(1):309–315. [PubMed] [Google Scholar]
- Bhattacharya G. Effects of metal ions on the utilization of glucose and on the influence of insulin on it by the isolated rat diaphragm. Biochem J. 1961 May;79(2):369–377. doi: 10.1042/bj0790369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARLIN H., HECHTER O. The disulfide-sulfhydryl interchange as a mechanism of insulin action. J Biol Chem. 1962 Apr;237:1371–1372. [PubMed] [Google Scholar]
- DESCHEPPER P. J., TOYODA M., BESSMAN S. P. A REQUIREMENT FOR CARBOHYDRATE METABOLISM FOR THE STIMULATION OF AMINO ACID INCORPORATION INTO PROTEIN BY INSULIN. J Biol Chem. 1965 Apr;240:1670–1674. [PubMed] [Google Scholar]
- FISHER R. B., WILLIAMSON J. R. The effects of insulin, adrenaline and nutrients on the oxygen uptake of the perfused rat heart. J Physiol. 1961 Sep;158:102–112. doi: 10.1113/jphysiol.1961.sp006757. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FONG C. T., SILVER L., POPENOE E. A., DEBONS A. F. Some observations on insulin-receptor interaction. Biochim Biophys Acta. 1962 Jan 1;56:190–192. doi: 10.1016/0006-3002(62)90551-6. [DOI] [PubMed] [Google Scholar]
- HARRIS E. J. THE DEPENDENCE OF EFFLUX OF SODIUM FROM FROG MUSCLE ON INTERNAL SODIUM AND EXTERNAL POTASSIUM. J Physiol. 1965 Apr;177:355–376. doi: 10.1113/jphysiol.1965.sp007597. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARRIS E. J. The site of swelling in muscle. J Biophys Biochem Cytol. 1961 Feb;9:502–504. doi: 10.1083/jcb.9.2.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENDLER R. W. A model for protein synthesis. Nature. 1962 Mar 3;193:821–823. doi: 10.1038/193821a0. [DOI] [PubMed] [Google Scholar]
- KIPNIS D. M., CORI C. F. Studies of tissue permeability. III. The effect of insulin on pentose uptake by the diaphragm. J Biol Chem. 1957 Feb;224(2):681–693. [PubMed] [Google Scholar]
- KIPNIS D. M., CORI C. F. Studies of tissue permeability. V. The penetration and phosphorylation of 2-deoxyglucose in the rat diaphragm. J Biol Chem. 1959 Jan;234(1):171–177. [PubMed] [Google Scholar]
- KIPNIS D. M., NOALL M. W. Stimulation of amino acid transport by insulin in the isolated rat diaphragm. Biochim Biophys Acta. 1958 Apr;28(1):226–227. doi: 10.1016/0006-3002(58)90466-9. [DOI] [PubMed] [Google Scholar]
- KIPNIS D. M., REISS E., HELMREICH E. Functional heterogeneity of the intracellular amino acid pool in mammalian cells. Biochim Biophys Acta. 1961 Aug 19;51:519–524. doi: 10.1016/0006-3002(61)90608-4. [DOI] [PubMed] [Google Scholar]
- KOSTYO J. L. SEPARATION OF THE EFFECTS OF GROWTH HORMONE ON MUSCLE AMINO ACID TRANSPORT AND PROTEIN SYNTHESIS. Endocrinology. 1964 Jul;75:113–119. doi: 10.1210/endo-75-1-113. [DOI] [PubMed] [Google Scholar]
- KRAHL M. E. Speculations on the action of insulin, with a note on other hypoglycemic agents. Perspect Biol Med. 1957;1(1):69–96. doi: 10.1353/pbm.1957.0000. [DOI] [PubMed] [Google Scholar]
- KREBS H. A., EGGLESTON L. V., D'ALESSANDRO A. The effect of succinate and amytal on the reduction of acetoacetate in animal tissues. Biochem J. 1961 Jun;79:537–549. doi: 10.1042/bj0790537. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KULKA R. G., KREBS H. A., EGGLESTON L. V. The reduction of acetoacetate to beta-hydroxybutyrate in animal tissues. Biochem J. 1961 Jan;78:95–106. doi: 10.1042/bj0780095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANCHESTER K. L. Insulin and incorporation of amino acids into protein of muscle. Cellular amino acid levels and aminoisobutyric acid uptake. Biochem J. 1961 Oct;81:135–147. doi: 10.1042/bj0810135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANCHESTER K. L., WOOL I. G. INSULIN AND INCORPORATION OF AMINO ACIDS INTO PROTEIN OF MUSCLE. 1. ACCUMULATION AND INCORPORATION STUDIES WITH THE PERFUSED RAT HEART. Biochem J. 1963 Nov;89:202–209. doi: 10.1042/bj0890202. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANCHESTER K. L., YOUNG F. G. Hormones and protein biosynthesis in isolated rat diaphragm. J Endocrinol. 1959 Jul;18:381–394. doi: 10.1677/joe.0.0180381. [DOI] [PubMed] [Google Scholar]
- MANCHESTER K. L., YOUNG F. G. The effect of insulin in vitro on the accumulation of amino acids by isolated rat diaphragm. Biochem J. 1960 Jun;75:487–495. doi: 10.1042/bj0750487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MIRSKY I. A., PERISUTTI G. The inhibition of the action of insulin on rat epididymal adipose tissue by sulfhydryl blocking agents. Biochim Biophys Acta. 1962 Aug 27;62:490–496. doi: 10.1016/0006-3002(62)90229-9. [DOI] [PubMed] [Google Scholar]
- OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
- PARRISH J. E., KIPNIS D. M. EFFECT OF NA ON SUGAR AND AMINO ACID TRANSPORT IN STRIATED MUSCLE. J Clin Invest. 1964 Oct;43:1994–2002. doi: 10.1172/JCI105073. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PECKHAM W. D., KNOBIL E. Amino acid concentration by the rat diaphragm in response to injury and to some metabolic inhibitors. Biochim Biophys Acta. 1962 Sep 10;63:207–209. doi: 10.1016/0006-3002(62)90356-6. [DOI] [PubMed] [Google Scholar]
- PETERS R. A. Hormones and the cytoskeleton. Nature. 1956 Mar 3;177(4505):426–426. doi: 10.1038/177426a0. [DOI] [PubMed] [Google Scholar]
- SIEKEVITZ P., POTTER V. R. Biochemical structure of mitochondria. II. Radioactive labeling of intra-mitochondrial nucleotides during oxidative phosphorylation. J Biol Chem. 1955 Jul;215(1):237–255. [PubMed] [Google Scholar]
- VIDAVER G. A. GLYCINE TRANSPORT BY HEMOLYZED AND RESTORED PIGEON RED CELLS. Biochemistry. 1964 Jun;3:795–799. doi: 10.1021/bi00894a011. [DOI] [PubMed] [Google Scholar]
- VILLEE C. A., WHITE V. K., HASTINGS A. B. Metabolism of C14 labeled glucose and pyruvate by rat diaphragm muscle in vitro. J Biol Chem. 1952 Mar;195(1):287–297. [PubMed] [Google Scholar]
- WILLIAMSON J. R., KREBS H. A. Acetoacetate as fuel of respiration in the perfused rat heart. Biochem J. 1961 Sep;80:540–547. doi: 10.1042/bj0800540. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOODWARD G. E., HUDSON M. T. The effect of 2-desoxy-D-glucose on glycolysis and respiration of tumor and normal tissues. Cancer Res. 1954 Sep;14(8):599–605. [PubMed] [Google Scholar]
- WOOL I. G. Stimulation of C14-histidine incorporation into protein of isolated diaphragm by nonutilized sugars. Am J Physiol. 1960 Jan;198:57–59. doi: 10.1152/ajplegacy.1960.198.1.57. [DOI] [PubMed] [Google Scholar]
- Wool I. G., Castles J. J., Moyer A. N. Regulation of amino acid accumulation in isolated rat diagphragm: effect of puromycin and insulin. Biochim Biophys Acta. 1965 Sep 13;107(2):333–345. doi: 10.1016/0304-4165(65)90139-x. [DOI] [PubMed] [Google Scholar]