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. 1966 Mar;98(3):898–902. doi: 10.1042/bj0980898

The tricarboxylic acid cycle and glycolysis in relation to ion transport by the ciliary body

M V Riley 1
PMCID: PMC1264934  PMID: 5911534

Abstract

1. The respiration and aerobic glycolysis of pig ciliary processes in oxygenated phosphate and bicarbonate buffers have been investigated. 2. Significant amounts of lactic acid are produced only in the presence of added glucose, but this does not change the endogenous respiration rate. 3. Succinate and citrate increase the oxygen uptake considerably, but pyruvate has almost no effect; oxaloacetate and fumarate stimulate slightly in the presence of glucose. Aspartate and fumarate together stimulate pyruvate utilization and are oxidized as fast as citrate. 4. Ouabain inhibits the oxidation of glucose and other substrates by limiting the ADP supply from the sodium transport system. Cyanide and azide inhibit respiration and stimulate glycolysis. 5. The transport mechanism depends largely on ATP from oxidative phosphorylation and regulates the rate of respiration and glycolysis by controlling ADP production from the Na+–K+-activated adenosine triphosphatase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cole D. F. ELECTRICAL POTENTIAL ACROSS THE ISOLATED CILIARY BODY OBSERVED IN VITRO. Br J Ophthalmol. 1961 Oct;45(10):641–653. doi: 10.1136/bjo.45.10.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cole D. F. ELECTROCHEMICAL CHANGES ASSOCIATED WITH THE FORMATION OF THE AQUEOUS HUMOUR. Br J Ophthalmol. 1961 Mar;45(3):202–217. doi: 10.1136/bjo.45.3.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELSHOVE A., VAN ROSSUMG NET MOVEMENTS OF SODIUM AND POTASSIUM, AND THEIR RELATION TO RESPIRATION, IN SLICES OF RAT LIVER INCUBATED IN VITRO. J Physiol. 1963 Oct;168:531–553. doi: 10.1113/jphysiol.1963.sp007206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HATEFI Y., JURTSHUK P., HAAVIK A. G. Studies on the electron transport system. 32. Respiratory control in beef heart mitochondria. Arch Biochem Biophys. 1961 Jul;94:148–155. doi: 10.1016/0003-9861(61)90022-4. [DOI] [PubMed] [Google Scholar]
  5. KINOSHITA J. H. The stimulation of the phosphogluconate oxidation pathway by pyruvate in bovine corneal epithelium. J Biol Chem. 1957 Sep;228(1):247–253. [PubMed] [Google Scholar]
  6. KLAHR S., BRICKER N. S. ENERGETICS OF ANAEROBIC SODIUM TRANSPORT BY THE FRESH WATER TURTLE BLADDER. J Gen Physiol. 1965 Mar;48:571–580. doi: 10.1085/jgp.48.4.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MARKS V. An improved glucose-oxidase method for determining blood, C.S.F. and urine glucose levels. Clin Chim Acta. 1959 May;4(3):395–400. doi: 10.1016/0009-8981(59)90110-x. [DOI] [PubMed] [Google Scholar]
  8. QUASTEL J. H., BICKIS I. J. Metabolism of normal tissues and neoplasms in vitro. Nature. 1959 Jan 31;183(4657):281–286. doi: 10.1038/183281a0. [DOI] [PubMed] [Google Scholar]
  9. RILEY M. V., BARONCELLI V. METABOLISM OF CILIARY BODY IN RELATION TO FORMATION OF AQUEOUS HUMOUR. Nature. 1964 Feb 8;201:621–621. doi: 10.1038/201621a0. [DOI] [PubMed] [Google Scholar]
  10. RILEY M. V. INHIBITION BY OUABAIN OF GLYCOLYSIS IN CILIARY BODY. Nature. 1964 Oct 24;204:380–380. doi: 10.1038/204380a0. [DOI] [PubMed] [Google Scholar]
  11. SCULLICA L. Observations of uveal blood flow pattern in excised arterially perfused rabbit eyes. Am J Ophthalmol. 1962 Dec;54:1057–1064. [PubMed] [Google Scholar]
  12. WHITTAM R., AGER M. E., WILEY J. S. CONTROL OF LACTATE PRODUCTION BY MEMBRANE ADENOSINE TRIPHOSPHATASE ACTIVITY IN HUMAN ERYTHROCYTES. Nature. 1964 Jun 13;202:1111–1112. doi: 10.1038/2021111a0. [DOI] [PubMed] [Google Scholar]
  13. WHITTAM R., WILLIS J. S. ION MOVEMENTS AND OXYGEN CONSUMPTION IN KIDNEY CORTEX SLICES. J Physiol. 1963 Aug;168:158–177. doi: 10.1113/jphysiol.1963.sp007184. [DOI] [PMC free article] [PubMed] [Google Scholar]

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