Abstract
1. The precipitation of the proteins of calf-thymus nucleohistone and herringsperm nucleoprotamine with the salts of fluorine-containing carboxylic acids has been studied. Pentadecafluoro-octanoate gave a precipitate that could be sedimented almost completely by centrifuging at 2350g for 20min. ω-H-Hexadecafluorononanoate, ω-H-eicosafluoro-undecanoate and dodecyl sulphate gave precipitates that could only be partly sedimented under these conditions. 2. A method has been developed for the isolation of highly polymerized DNA on a large scale from calf thymus and herring sperm by the use of pentadecafluorooctanoate. This is more convenient and less tedious than existing methods.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- KIRBY K. S. A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem J. 1957 Jul;66(3):495–504. doi: 10.1042/bj0660495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARKO A. M., BUTLER G. C. The isolation of sodium desoxyribonucleate with sodium dodecyl sulfate. J Biol Chem. 1951 May;190(1):165–176. [PubMed] [Google Scholar]
- Mirsky A. E., Pollister A. W. Nucleoproteins of Cell Nuclei. Proc Natl Acad Sci U S A. 1942 Sep;28(9):344–352. doi: 10.1073/pnas.28.9.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WYATT G. R., COHEN S. S. The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine. Biochem J. 1953 Dec;55(5):774–782. doi: 10.1042/bj0550774. [DOI] [PMC free article] [PubMed] [Google Scholar]