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. 1966 Apr;99(1):228–231. doi: 10.1042/bj0990228

The fate of acetyl groups derived from glucose in the isolated perfused goat udder

D C Hardwick 1
PMCID: PMC1264979  PMID: 5965339

Abstract

1. In the isolated perfused goat mammary gland, both glucose and acetate contribute 20–30% of carbon to citrate via acetyl-CoA. 2. Carbon dioxide does not contribute significantly to the formation of acetyl-CoA. 3. ATP citrate lyase, which, in rats, yields acetyl-CoA for fatty acid synthesis, is absent from or very low in high-speed supernatant fraction of ruminant tissue. 4. It is reasonable to infer that intramitochondrial acetyl-CoA, to which both glucose and acetate contribute, is separated from extramitochondrial acetyl-CoA to which glucose does not contribute and which is used for fatty acid synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANNISON E. F. Studies on the volatile fatty acids of sheep blood with special reference to formic acid. Biochem J. 1954 Dec;58(4):670–680. doi: 10.1042/bj0580670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BACH S. J., HIBBITT K. G. Biochemical aspects of bovine ketosis. Biochem J. 1959 May;72(1):87–92. doi: 10.1042/bj0720087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartley J., Abraham S., Chaikoff I. L. Concerning the form in which acetyl units produced in mitochondria are transferred to the site of de novo fatty acid synthesis in the cell. Biochem Biophys Res Commun. 1965 Jun 9;19(6):770–776. doi: 10.1016/0006-291x(65)90326-8. [DOI] [PubMed] [Google Scholar]
  4. HARDWICK D. C. THE INCORPORATION OF CARBON DIOXIDE INTO MILK CITRATE IN THE ISOLATED PERFUSED GOAT UDDER. Biochem J. 1965 Apr;95:233–237. doi: 10.1042/bj0950233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hardwick D. C., Linzell J. L., Mepham T. B. The metabolism of acetate and glucose by the isolated perfused udder. 2. The contribution of acetate and glucose to carbon dioxide and milk constituents. Biochem J. 1963 Aug;88(2):213–220. doi: 10.1042/bj0880213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KORNACKER M. S., LOWENSTEIN J. M. CITRATE AND THE CONVERSION OF CARBOHYDRATE INTO FAT. THE ACTIVITIES OF CITRATE-CLEAVAGE ENZYME AND ACETATE THIOKINASE IN LIVERS OF STARVED AND RE-FED RATS. Biochem J. 1965 Jan;94:209–215. doi: 10.1042/bj0940209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. NOVELLI G. D. Methods for determination of coenzyme A. Methods Biochem Anal. 1955;2:189–213. doi: 10.1002/9780470110188.ch7. [DOI] [PubMed] [Google Scholar]
  8. PIGRETTI M. M., STOPPANI A. O. Chemical degradation of 14C-labeled glutamic acid. Biochim Biophys Acta. 1961 Sep 16;52:390–392. doi: 10.1016/0006-3002(61)90693-x. [DOI] [PubMed] [Google Scholar]
  9. SHIPP J. C., MATOS O. E., KNIZLEY H., CREVASSE L. E. CO-2 FORMED FROM ENDOGENOUS AND EXOGENOUS SUBSTRATES IN PERFUSED RAT HEART. Am J Physiol. 1964 Dec;207:1231–1236. doi: 10.1152/ajplegacy.1964.207.6.1231. [DOI] [PubMed] [Google Scholar]
  10. SPENCER A. F., LOWENSTEIN J. M. The supply of precursors for the synthesis of fatty acids. J Biol Chem. 1962 Dec;237:3640–3648. [PubMed] [Google Scholar]
  11. SRERE P. A., BHADURI A. Incorporation of radioactive citrate into fatty acids. Biochim Biophys Acta. 1962 May 21;59:487–489. doi: 10.1016/0006-3002(62)90205-6. [DOI] [PubMed] [Google Scholar]
  12. SRERE P. A. The citrate cleavage enzyme. I. Distribution and purification. J Biol Chem. 1959 Oct;234:2544–2547. [PubMed] [Google Scholar]
  13. STADTMAN E. R., BARKER H. A. Fatty acid synthesis by enzyme preparations of Clostridium kluyveri. VI. Reactions of acyl phosphates. J Biol Chem. 1950 Jun;184(2):769–793. [PubMed] [Google Scholar]
  14. Spencer A., Corman L., Lowenstein J. M. Citrate and the conversion of carbohydrate into fat. A comparison of citrate and acetate incorporation into fatty acids. Biochem J. 1964 Nov;93(2):378–388. doi: 10.1042/bj0930378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TOMBROPOULOS E. G., KLEIBER M. The metabolism of specifically labelled glucose in bovine ketosis. Biochem J. 1961 Aug;80:414–420. doi: 10.1042/bj0800414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WEINMAN E. O., STRISOWER E. H., CHAIKOFF I. L. Conversion of fatty acids to carbohydrate; application of isotopes to this problem and role of the Krebs cycle as a synthetic pathway. Physiol Rev. 1957 Apr;37(2):252–272. doi: 10.1152/physrev.1957.37.2.252. [DOI] [PubMed] [Google Scholar]

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