Abstract
1. The incorporation of 14CO2 and dl-[2-14C]mevalonic acid into various terpenoids in developing chloroplasts in a number of seedlings has been studied. 2. β-Carotene and phytol (from chlorophyll) tend to be heavily labelled from 14CO2, whereas sterols and β-amyrin are only slightly labelled; with dl-[2-14C]mevalonic acid the situation is reversed. 3. The incorporation of 14CO2 into terpenoids is dependent on the stage of chloroplast development, whereas that of mevalonic acid is independent of chloroplast development. 4. The uptake of 14CO2 into β-carotene and phytol in mature chloroplasts is very low in monocotyledons but somewhat greater in dicotyledons. 5. The results are discussed in relation to the view that terpenoid biosynthesis in developing chloroplasts is regulated by a combination of enzyme segregation and specific membrane permeability.
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Selected References
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- Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mercer E. I., Davies B. H., Goodwin T. W. Studies in carotenogenesis. 29. Attempts to detect lycopersene in higher plants. Biochem J. 1963 May;87(2):317–325. doi: 10.1042/bj0870317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PERKINS H. J., ROBERTS D. W. Purification of chlorophylls, pheophytins and pheophorbides for specific activity determinations. Biochim Biophys Acta. 1962 Apr 23;58:486–498. doi: 10.1016/0006-3002(62)90059-8. [DOI] [PubMed] [Google Scholar]