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. 1966 Apr;99(1):246–252. doi: 10.1042/bj0990246

The enzymic estimation of glutamate and glutamine

Julia M Sowerby 1, J H Ottaway 1
PMCID: PMC1264982  PMID: 5965342

Abstract

A method of estimating glutamic acid is described, based on its dehydrogenation by glutamate dehydrogenase coupled, by means of N-methylphenazine methosulphate, to the reduction of tetrazolium salts. The method is suitable for the estimation of 0–0·3μmole of glutamic acid. The response is linear, but not stoicheiometric: possible reasons for this are discussed. If suitable precautions are taken, the use of a partially purified preparation of glutaminase makes it possible to estimate glutamine also.

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Selected References

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