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. 1966 May;99(2):381–388. doi: 10.1042/bj0990381

Intracellular localization of mevalonate-activating enzymes in plant cells

L J Rogers 1, S P J Shah 1, T W Goodwin 1
PMCID: PMC1265006  PMID: 5947149

Abstract

Mevalonate-activating enzymes are shown to be present in the chloroplasts of French-bean leaves. The chloroplast membrane is impermeable to mevalonic acid. Mevalonate-activating enzymes also appear to be found outside the chloroplast. These results support the view that terpenoid biosynthesis in the plant cell is controlled by a combination of enzyme segregation and specific membrane permeability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOCH K., CHAYKIN S., PHILLIPS A. H., DE WAARD A. Mevalonic acid pyrophosphate and isopentenylpyrophosphate. J Biol Chem. 1959 Oct;234:2595–2604. [PubMed] [Google Scholar]
  2. GOODWIN T. W. Studies in carotenogenesis. 25. The incorporation of 14CO2, [2-14C] acetate and [2-14C]mevalonate into beta-carotene by illuminated etiolated maize seedings. Biochem J. 1958 Dec;70(4):612–617. doi: 10.1042/bj0700612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HENNING U., MOSLEIN E. M., LYNEN F. Biosynthesis of terpenes. V. Formation of 5-pyrophosphomevalonic acid by phosphomevalonic kinase. Arch Biochem Biophys. 1959 Jul;83(1):259–267. doi: 10.1016/0003-9861(59)90031-1. [DOI] [PubMed] [Google Scholar]
  4. LEECH R. M. THE ISOLATION OF STRUCTURALLY INTACT CHLOROPLASTS. Biochim Biophys Acta. 1964 May 25;79:637–639. doi: 10.1016/0926-6577(64)90235-9. [DOI] [PubMed] [Google Scholar]
  5. Loomis W. D. Amide Metabolism in Higher Plants. III. Distribution of Glutamyl Tranferase and Glutamine Synthetase Activity. Plant Physiol. 1959 Sep;34(5):541–546. doi: 10.1104/pp.34.5.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. OHNOKI D., SUZUE G., TANAKA S. D5-Phosphomevalonic acid as the intermediate of the enzymatic synthesis of bacterial phytoene. J Biochem. 1962 Dec;52:423–427. doi: 10.1093/oxfordjournals.jbchem.a127638. [DOI] [PubMed] [Google Scholar]
  7. Williamson I. P., Kekwick R. G. The formation of 5-phosphomevalonate by mevalonate kinase in Hevea brasiliensis latex. Biochem J. 1965 Sep;96(3):862–871. doi: 10.1042/bj0960862. [DOI] [PMC free article] [PubMed] [Google Scholar]

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