Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1966 May;99(2):501–506. doi: 10.1042/bj0990501

A comparison of aminoacyl-β-naphthylamide hydrolases in extracts of human tissues

D K Panveliwalla 1, D W Moss 1
PMCID: PMC1265019  PMID: 5947151

Abstract

1. The aminoacyl-β-naphthylamide-hydrolase activities of extracts of human liver, kidney, pancreas and small intestine were partially purified by chromatography on DEAE-Sephadex. 2. The enzymes from the different tissues showed slight variations in chromatographic behaviour and in mobility on starch-gel electrophoresis. 3. Michaelis constants for the hydrolysis of l-leucyl- and l-alanyl-β-naphthylamide by the partially purified enzyme fractions were determined by a spectrofluorimetric assay method. The enzymes from different sources gave similar Km values. 4. l-Leucinamide and l-leucylglycine were competitive inhibitors of the hydrolysis of both substrates. Values of Ki (leucinamide) and Ki (leucylglycine) respectively were constant whatever combination of enzyme and substrate was used. 5. The relevance of these results to the identity or non-identity of the enzymes from different tissues is discussed.

Full text

PDF
501

Images in this article

503Fig. 2.
on p.503

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEHAL F. J., HAMILTON R. D., KANAVAGE C. B., KELLY E. C. Studies on aminopeptidases in human blood. Arch Biochem Biophys. 1963 Feb;100:308–312. doi: 10.1016/0003-9861(63)90078-x. [DOI] [PubMed] [Google Scholar]
  2. Behal F. J., Asserson B., Dawson F., Hardman J. A study of human tissue aminopeptidase components. Arch Biochem Biophys. 1965 Aug;111(2):335–344. doi: 10.1016/0003-9861(65)90194-3. [DOI] [PubMed] [Google Scholar]
  3. COTLOVE E., TRANTHAM H. V., BOWMAN R. L. An instrument and method for automatic, rapid, accurate, and sensitive titration of chloride in biologic samples. J Lab Clin Med. 1958 Mar;51(3):461–468. [PubMed] [Google Scholar]
  4. FLEISHER G. A., PANKOW M., WARMKA C. LEUCINE AMINOPEPTIDASE IN HUMAN SERUM: COMPARISON OF HYDROLYSIS OF L-LEUCYLGLYCINE AND L-LEUCYL-BETA-NAPHTHYLAMIDE. Clin Chim Acta. 1964 Mar;9:259–268. doi: 10.1016/0009-8981(64)90105-6. [DOI] [PubMed] [Google Scholar]
  5. NAKAGAWA S., TSUJI H. ELECTROPHORETIC SEPARATION OF LEUCINE AMINOPEPTIDASE AND LEUCYL-BETA-NAPHTHYLAMIDE-SPLITTING ENZYME. Clin Chim Acta. 1964 Dec;10:572–573. doi: 10.1016/0009-8981(64)90200-1. [DOI] [PubMed] [Google Scholar]
  6. PATTERSON E. K., HSIAO S. H., KEPPEL A. STUDIES ON DIPEPTIDASES AND AMINOPEPTIDASES. I. DISTINCTION BETWEEN LEUCINE AMINOPEPTIDASE AND ENZYMES THAT HYDROLYZE L-LEUCYL-BETA-NAPHTHYLAMIDE. J Biol Chem. 1963 Nov;238:3611–3620. [PubMed] [Google Scholar]
  7. POULIK M. D. Starch gel electrophoresis in a discontinous system of buffers. Nature. 1957 Dec 28;180(4600):1477–1479. doi: 10.1038/1801477a0. [DOI] [PubMed] [Google Scholar]
  8. ROTH M. UNE ULTRAMICRO-M'ETHODE FLUIRIM'ETRIQUE POUR LE DOSAGE DE LA LEUCINE-AMINOPEPTIDASE DANS LES LIQUIDES BIOLOGIQUES. Clin Chim Acta. 1964 May;9:448–453. doi: 10.1016/0009-8981(64)90082-8. [DOI] [PubMed] [Google Scholar]
  9. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES