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. 1966 Jun;99(3):657–666. doi: 10.1042/bj0990657

Release of enzymes from lysosomes by irradiation and the relation of lipid peroxide formation to enzyme release

E D Wills 1, A E Wilkinson 1
PMCID: PMC1265055  PMID: 5964962

Abstract

1. Acid phosphatase, cathepsin and β-glucuronidase are released from rat-liver lysosomes by irradiation in vitro. Enzyme release is detectable after a dose of 1krad and increases with dose up to 100krads. 2. Maximum radiation effects were observed when the lysosomes were kept for 20hr. at 4° or 20° after irradiation. 3. An atmosphere of nitrogen considerably decreases enzyme release from lysosomes. 4. Enzyme release is enhanced by ascorbic acid and decreased by vitamin E. 5. Irradiation causes formation of lipid peroxides in lysosomes, and enzyme release increases with lipid peroxide formation. 6. It is suggested that lipid peroxide formation leads to rupture of the lysosome membrane and allows release of the contained hydrolytic enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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