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. 1966 Jun;99(3):717–725. doi: 10.1042/bj0990717

A method for the estimation of urinary testosterone

A A A Ismail 1, R A Harkness 1
PMCID: PMC1265063  PMID: 5964968

Abstract

1. A method has been developed for the estimation of testosterone in human urine by using acid hydrolysis followed by a quantitative form of a modified Girard reaction that separates a `conjugated-ketone' fraction from a urine extract; this is followed by column chromatography on alumina and paper chromatography. 2. Comparison of methods of estimation of testosterone in the final fraction shows that estimation by gas–liquid chromatography is more reproducible than by colorimetric methods applied to the same eluates from the paper chromatogram. 3. The mean recovery of testosterone by gas–liquid chromatography is 79·5%, and this method appears to be specific for testosterone. 4. The procedure is relatively rapid. Six determinations can be performed by one worker in 2 days. 5. Results of determinations on human urine are briefly presented. In general, they are similar to earlier estimates, but the maximal values are lower.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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