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. 1966 Jul;100(1):131–137. doi: 10.1042/bj1000131

Amino acid incorporation into the protein of mitochondrial preparations from cerebral cortex and spinal cord

H S Bachelard 1,*
PMCID: PMC1265103  PMID: 5965248

Abstract

1. Washed guinea-pig cerebral-cortex mitochondria incorporate [14C]leucine into their protein at a rate comparable with the rates reported for liver or heart mitochondria only if the mitochondria are separated from myelin and nerve endings by density-gradient centrifugation. 2. The non-mitochondrial components (myelin and nerve endings) of brain mitochondrial preparations incorporated [14C]leucine at a negligible rate. 3. The mitochondria do not require an exogenous supply of energy or a full supply of amino acids to support the process. 4. The incorporation rate was linear up to 2hr. aerobic incubation at 30° and was inhibited by chloramphenicol, only slightly by actinomycin D and not by penicillin or pretreatment with ribonuclease. The observed incorporation is considered to be unlikely to be due to contaminating cytoplasmic ribosomes or bacteria. 5. The process was also studied in mitochondrial preparations from rabbit cerebral cortex and spinal cord.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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