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. 1966 Aug;100(2):303–308. doi: 10.1042/bj1000303

The N-terminal sequence of the heavy chain of rabbit immunoglobulin IgG

J M Wilkinson 1, E M Press 1, R R Porter 1
PMCID: PMC1265136  PMID: 4165510

Abstract

The absence of an N-terminal amino acid with a free α-amino group from the heavy chain of rabbit immunoglobulin IgG has been confirmed and no evidence could be found of a blocking formyl, acetyl or propionyl group. The N-terminal amino acid appears to be pyrrolid-2-one-5-carboxylic acid (PCA) in all molecules. A mixed amino acid sequence follows in the approximate proportions: PCA-Ser-Val-Glu-Glu-Ser-Gly-Gly-Arg, 50%; PCA-Ser-Leu-Glu, 20%; PCA-Glu(NH2), 20%. The heavy chains of a purified antibody, namely anti-(human serum albumin), and of immunoglobulin IgG from a rabbit homozygous at the allotypic loci both showed a similar mixed N-terminal sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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