Abstract
1. An account is given of the development of a rapid method for separating water-soluble N-2,4-dinitrophenylamino acids by liquid–liquid partition chromatography. 2. The mechanism of the chromatography is discussed. 3. The R values of a number of water-soluble and ether-soluble DNP derivatives are listed.
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Selected References
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- BETTELHEIM F. R. Amino terminal group in chymotrypsinogen. J Biol Chem. 1955 Jan;212(1):235–239. [PubMed] [Google Scholar]
- CLOSE J., ADRIAENS E. L., MOORE S., BIGWOOD E. J. Composition en acides aminés d'hydrolysats de farine de manioc roul variété amère. Bull Soc Chim Biol (Paris) 1953;35(9):985–992. [PubMed] [Google Scholar]
- EASTOE J. E., KENCHINGTON A. W. Ion-exchange chromatography of guanidino amino-acids from synthetically modified gelatin. Nature. 1954 Nov 20;174(4438):966–967. doi: 10.1038/174966a0. [DOI] [PubMed] [Google Scholar]
- HAMILTON P. B., ANDERSON R. A. Demonstration of ornithine in gelatin by ion exchange chromatography. J Biol Chem. 1954 Nov;211(1):95–102. [PubMed] [Google Scholar]
- HIRS C. H. Dinitrophenyiribonucleases. Brookhaven Symp Biol. 1962 Dec;15:154–183. [PubMed] [Google Scholar]
- Hirs C. H., Halmann M., Kycia J. H. Dinitrophenylation and inactivation of bovine pancreatic ribonuclease A. Arch Biochem Biophys. 1965 Jul;111(1):209–222. doi: 10.1016/0003-9861(65)90343-7. [DOI] [PubMed] [Google Scholar]
- MATHESON N. A. An improved method of separating amino acids as N-2,4-dinitrophenyl derivatives. Biochem J. 1963 Jul;88:146–151. doi: 10.1042/bj0880146. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MATHESON N. A. THE ISOLATION OF AMINO ACIDS FROM MIXTURES AS THEIR N-2,4-DINITROPHENYL DERIVATIVES, METHIONINE, LEUCINE, PHENYLALANINE, ORNITHINE, LYSINE AND TYROSINE. Biochem J. 1965 Mar;94:513–517. doi: 10.1042/bj0940513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matheson N. A., Sheltawy M. Determination of amino acids as 2,4-dinitrophenyl derivatives. Serine and lysine. Biochem J. 1966 Jan;98(1):297–302. doi: 10.1042/bj0980297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOZAKI Y., TANFORD C. THE SOLUBILITY OF AMINO ACIDS AND RELATED COMPOUNDS IN AQUEOUS UREA SOLUTIONS. J Biol Chem. 1963 Dec;238:4074–4081. [PubMed] [Google Scholar]
- Porter R. R., Sanger F. The free amino groups of haemoglobins. Biochem J. 1948;42(2):287–294. doi: 10.1042/bj0420287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUSSELL D. W. Studies on the photochemical behavior of 2,4-dinitrophenyl derivatives of some amino acids and peptides. Biochem J. 1963 Apr;87:1–4. doi: 10.1042/bj0870001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIEPMANN E., ZAHN H. NIM-2,4-DINITROPHENYL-L-HISTIDIN. Biochim Biophys Acta. 1964 Feb 10;82:412–415. [PubMed] [Google Scholar]
- ZAHN H., UMLAUF E. Uber Oxylysin. Hoppe Seylers Z Physiol Chem. 1954;297(3-6):127–145. [PubMed] [Google Scholar]
- ZAHN H., WURZ A. Uber die Reaktion des Seidenfibroins mit 2, 4-Dinitrofluorbenzol. Biochem Z. 1952 Mar;322(5):327–339. [PubMed] [Google Scholar]
