Abstract
A method has been developed for separation and partial purification of four protein hormones from acetone-dried human pituitary glands. The major portion of growth hormone, follicle-stimulating hormone, luteinizing hormone and thyroid-stimulating hormone activities are each obtained in a separate fraction. Although the yields of the individual hormones are similar to those obtained by other investigators, the present procedure is simpler and a cleaner separation of the hormones has been achieved. Methods for further purification of each fraction are indicated including preparation of luteinizing hormone of very high potency (equivalent to 5·3mg. of standard NIH–LH–S3/mg.).
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAKKE J. L. ASSAY OF HUMAN THYROID-STIMULATING HORMONE BY 18 DIFFERENT ASSAY LABORATORIES USING 12 DIFFERENT METHODS. J Clin Endocrinol Metab. 1965 Apr;25:545–550. doi: 10.1210/jcem-25-4-545. [DOI] [PubMed] [Google Scholar]
- BATES R. W., CONDLIFFE P. G. Studies on the chemistry and bioassay of thyrotropins from bovine pituitaries, transplantable pituitary tumors of mice, and blood plasma. Recent Prog Horm Res. 1960;16:309–352. [PubMed] [Google Scholar]
- BUTT W. R., CROOKE A. C., CUNNINGHAM F. J., EVANS A. J. Chemical reactions which affect the biological activity of human gonadotrophins. Biochem J. 1961 Apr;79:64–70. doi: 10.1042/bj0790064. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BUTT W. R., CROOKE A. C., CUNNINGHAM F. J. Studies on human urinary and pituitary gonadotrophins. Biochem J. 1961 Dec;81:596–605. doi: 10.1042/bj0810596. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BUTT W. R., CUNNINGHAM F. J., HARTREE A. S. GONADOTROPHINS. PREPARATION AND ASSAY OF HUMAN PITUITARY FSH AND LH. Proc R Soc Med. 1964 Feb;57:107–108. doi: 10.1177/003591576405700213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CONDLIFFE P. G. Purification of human thyrotrophin. Endocrinology. 1963 Jun;72:893–896. doi: 10.1210/endo-72-6-893. [DOI] [PubMed] [Google Scholar]
- EHRLICH R. M., RANDLE P. J. Immunoassay of growth hormone in human serum. Lancet. 1961 Jul 29;2(7196):230–233. doi: 10.1016/s0140-6736(61)90355-5. [DOI] [PubMed] [Google Scholar]
- HARTREE A. S., BUTT W. R., KIRKHAM K. E. THE SEPARATION AND PURIFICATION OF HUMAN LUTEINIZING AND THYROTROPHIC HORMONES. J Endocrinol. 1964 Apr;29:61–69. doi: 10.1677/joe.0.0290061. [DOI] [PubMed] [Google Scholar]
- Hartree A. S., Kovacić N., Thomas M. Growth-promoting and luteotrophic activities of human growth hormone. J Endocrinol. 1965 Oct;33(2):249–258. doi: 10.1677/joe.0.0330249. [DOI] [PubMed] [Google Scholar]
- KIRKHAM K. E. A new bioassay technique for the measurement in vitro of thyrotrophic hormone in serum and in pituitary extracts. J Endocrinol. 1962 Oct;25:259–269. doi: 10.1677/joe.0.0250259. [DOI] [PubMed] [Google Scholar]
- PARLOW A. F., CONDLIFFE P. G., REICHERT L. E., Jr, WILHELMI A. E. RECOVERY AND PARTIAL PURIFICATION OF FSH AND LH DURING THE PURIFICATION OF TSH FROM HUMAN PITUITARY GLANDS. Endocrinology. 1965 Jan;76:27–34. doi: 10.1210/endo-76-1-27. [DOI] [PubMed] [Google Scholar]
- Parlow A. F., Wilhelmi A. E., Reichert L. E., Jr Further studies on the fractionation of human pituitary glands. Endocrinology. 1965 Dec;77(6):1126–1134. doi: 10.1210/endo-77-6-1126. [DOI] [PubMed] [Google Scholar]
- READ C. H., BRYAN G. T. The immunological assay of human growth hormone. Recent Prog Horm Res. 1960;16:187–218. [PubMed] [Google Scholar]
- REICHERT L. E., Jr, PARLOW A. F. PARTIAL PURIFICATION AND SEPARATION OF HUMAN PITUITARY GONADOTROPHINS. Endocrinology. 1964 Feb;74:236–243. doi: 10.1210/endo-74-2-236. [DOI] [PubMed] [Google Scholar]
- REICHERT LE, Jr, PARLOW A. F. FURTHER STUDIES ON THE PURIFICATION OF HUMAN PITUITARY LUTEINIZING HORMONES. Endocrinology. 1964 Nov;75:815–817. doi: 10.1210/endo-75-5-815. [DOI] [PubMed] [Google Scholar]
- STEELMAN S. L., SEGALOFF A., ANDERSEN R. N. Purification of human pituitary follicle stimulating (FSH) and luteinizing (LH) hormones. Proc Soc Exp Biol Med. 1959 Jul;101(3):452–454. doi: 10.3181/00379727-101-24976. [DOI] [PubMed] [Google Scholar]
- STEELMAN S. L., SEGALOFF A., MAYS M. G. Preparation of highly active human gonadotropin and growth hormone. Arch Biochem Biophys. 1958 Nov;78(1):262–263. doi: 10.1016/0003-9861(58)90337-0. [DOI] [PubMed] [Google Scholar]
- WILHELMI A. E. Fractionation of human pituitary glands. Can J Biochem Physiol. 1961 Nov;39:1659–1668. doi: 10.1139/o61-183. [DOI] [PubMed] [Google Scholar]
