Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1966 Sep;100(3):775–778. doi: 10.1042/bj1000775

Proteolytic and peptidase activities of the jejunum and ileum of the rat during postnatal development

R Noack 1, O Koldovský 1, M Friedrich 1, A Heringová 1, V Jirsová 1, G Schenk 1
PMCID: PMC1265214  PMID: 5969290

Abstract

1. Proteolytic (substrate nitrocasein), tripeptidase (substrate glycylglycylgly-cine) and aminopeptidase (substrate leucyl-β-naphthylamide) activities were studied in homogenates of jejunal and ileal mucosa of 7-, 10-, 14-, 21-, 35- and 60-day-old rats. 2. Proteolytic activity was practically the same in jejunum of 7-, 10-, 14- and 21-day-old rats, but after day 21 a significant increase was observed. The activity of the ileum changed very little during postnatal development and was always higher than that of the jejunum. 3. Tripeptidase activity was low in the jejunum of 7- and 14-day-old rats, an increase was observed between day 14 and 21, but later no substantial changes were found. There were no changes in the ileum. The activity in the jejunum of 7- and 14-day-old rats was lower than in the ileum, but later the jejunum was more active than the ileum. 4. Aminopeptidase activity had a similar developmental pattern to tripeptidase activity. A low activity was found in the jejunum of 7- and 14-day-old rats, the maximum was in 21-day-old rats and then a decrease was observed, though values for 60-day-old rats were still higher than for 7- and 10-day-old rats. The activity in the ileum was practically the same in all age groups studied except in 14- and 21-day-old rats, where a transient peak was observed.

Full text

PDF
775

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALVAREZ A., SAS J. beta-Galactosidase changes in the developing intestinal tract of the rat. Nature. 1961 May 27;190:826–827. doi: 10.1038/190826b0. [DOI] [PubMed] [Google Scholar]
  2. DOELL R. G., KRETCHMER N. Studies of small intestine during development. I. Distribution and activity of beta-galactosidase. Biochim Biophys Acta. 1962 Aug 13;62:353–362. doi: 10.1016/0006-3002(62)90097-5. [DOI] [PubMed] [Google Scholar]
  3. Friedrich M., Noack R., Schenk G. Zur Lokalisation von peptidatischen und proteolytischen Aktvitäten in isolierten Bürstensäumen aus der Mucosa des Rattendünndarmes. Biochem Z. 1965 Dec 31;343(4):346–353. [PubMed] [Google Scholar]
  4. GREEN M. N., TSOU K. C., BRESSLER R., SELIGMAN A. M. The colorimetric determination of leucine aminopeptidase activity with L-leucyl-beta-naphthylamide hydrochloride. Arch Biochem Biophys. 1955 Aug;57(2):458–474. doi: 10.1016/0003-9861(55)90307-6. [DOI] [PubMed] [Google Scholar]
  5. HALLIDAY R. Prenatal and postnatal transmission of passive immunity to young rats. Proc R Soc Lond B Biol Sci. 1955 Nov 29;144(916):427–430. doi: 10.1098/rspb.1955.0068. [DOI] [PubMed] [Google Scholar]
  6. HALLIDAY R. The absorption of antibodies from immune sera by the gut of the young rat. Proc R Soc Lond B Biol Sci. 1955 Mar 15;143(912):408–413. doi: 10.1098/rspb.1955.0020. [DOI] [PubMed] [Google Scholar]
  7. HERINGOVA A., JIRSOVA V., KOLDOVSKY O. POSTNATAL DEVELOPMENT OF BETA-GLUCURONIDASE IN THE JEJUNUM AND ILEUM OF RATS. Can J Biochem. 1965 Feb;43:173–178. [PubMed] [Google Scholar]
  8. KOLDOVSKY O., CHYTIL F. POSTNATAL DEVELOPMENT OF BETA-GALACTOSIDASE ACTIVITY IN THE SMALL INTESTINE OF THE RAT. EFFECT OF ADRENALECTOMY AND DIET. Biochem J. 1965 Jan;94:266–270. doi: 10.1042/bj0940266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KOLDOVSKY O., HERINGOVA A., JIRSOVA V. ACTIVITY OF BETA-GLUCOSIDASE IN THE JEJUNUM AND ILEUM OF THE RAT DURING POSTNATAL DEVELOPMENT. Physiol Bohemoslov. 1965;14:228–232. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. MOSINGER B., PLACER Z., KOLDOVSKY O. Passage of insulin through the wall of the gastro-intestinal tract of the infant rat. Nature. 1959 Oct 17;184(Suppl 16):1245–1246. doi: 10.1038/1841245a0. [DOI] [PubMed] [Google Scholar]
  12. ROBINSON G. B., SHAW B. The hydrolysis of dipeptides by different regions of rat small intestine. Biochem J. 1960 Nov;77:351–356. doi: 10.1042/bj0770351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ROKOS J., HAHN P., KOLDVSKY O., PROCHAZKA P. The postnatal development of lipolytic activity in the pancreas and small intestine of the rat. Physiol Bohemoslov. 1963;12:213–219. [PubMed] [Google Scholar]
  14. RUBINO A., ZIMBALATTI F., AURICCHIO S. INTESTINAL DISACCHARIDASE ACTIVITIES IN ADULT AND SUCKLING RATS. Biochim Biophys Acta. 1964 Nov 22;92:305–311. doi: 10.1016/0926-6569(64)90187-7. [DOI] [PubMed] [Google Scholar]
  15. von PECHMANN E. Uber die enzymatische Hydrolyse von Xanthoproteinen und deren Verwendung zur colorimetrischen Bestimmung proteolytischer Fermente. Biochem Z. 1950;321(3):248–260. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES