Fig. 3.

Impact of miR-21 modulation on cell proliferation, apoptosis, cytokine secretion, and migration in P.g-LPS-treated HUVECs. (a) miR-21 levels were assessed by qRT-PCR after P.g-LPS treatment. (b) qRT-PCR results validating successful overexpression or knockdown of miR-21 following transfection with a mimic or inhibitor. (c–e) Cell viability and cytokine (IL-6 and TNF-α) secretion under different conditions. (f–g) Quantification of apoptosis using Annexin V/PI staining. (h, i) TUNEL staining was performed to quantify the proportion of apoptotic cells. (j, k) Wound healing assays were conducted to evaluate cell migration under different treatment conditions. Data are presented as mean ± standard deviation (n = 3). ∗P < 0.05. Results are representative of three independent experiments. Abbreviations: inhibitor NC = negative control for miR-21 inhibitor; inhibitor = miR-21 inhibitor; mimic NC = negative control for miR-21 mimic; mimic = miR-21 mimic.