Correction for “Synthetical lethality of Werner helicase and mismatch repair deficiency is mediated by p53 and PUMA in colon cancer,” by Suisui Hao, Jingshan Tong, Anupma Jha, Denise Risnik, Darleny Lizardo, Xinyan Lu, Ajay Goel, Patricia L. Opresko, Jian Yu, and Lin Zhang, which published December 12, 2022; 10.1073/pnas.2211775119 (Proc. Natl. Acad. Sci. U.S.A. 119, e2211775119).
The authors note that Fig. 5 appeared incorrectly. During figure assembly, the image for the active caspase 3 staining of CH3+5 +Dox sample in Fig. 5D was inadvertently duplicated into the image for active caspase 3 staining of CH3+5 -Dox sample. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 5.
Inducible knockdown of WRN suppresses xenograft growth in a PUMA-dependent fashion. Parental (WT), CH3+5, and PUMA-KO HCT116 cells with Dox inducible expression of WRN short hairpin RNA (Sh WRN) were established as described in Materials and Methods. (A) Western blotting of indicated proteins in two independent clones of WT, CH3+5, and PUMA-KO Sh WRN HCT116 cells with induction of Sh WRN by Dox (0.2 μg/mL) treatment for indicated time. (B) MTS analysis of viability of indicated cells with induction of Sh WRN as in (A) at indicated time points. Results were expressed as mean ± SD of three independent experiments. (C and D) Nude mice were implanted with 2 × 106 of WT (clone 1), CH3+5, or PUMA-KO Sh WRN HCT116 cells to establish xenograft tumors. Tumor bearing mice were randomized and treated with 18% Protein Rodent Diet ±Dox hyclate (625 mg/kg). (C) Tumor volume at indicated time points after treatment was measured and plotted with P values for indicated comparisons (N = 6 in each group). (D) Tumor sections from mice treated as in (C) for 13 d were analyzed by immunostaining for active caspase 3 expression. (Left) Representative staining pictures with arrows indicating examples of positive signals (Scale bars: 25 μm); (Right) Quantification of positive signals showing means ± SEM of each field in three fields per mouse (N = 3 in each group). *P < 0.05; **P < 0.01; ***P < 0.001.