A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography. Corrigendum

Abstract

Information concerning sample preparation in the article by Ghosh et al. [J. Appl. Cryst. (2023), 56, 449–460] is clarified.

In the article by Ghosh et al. (2023 ▸), the microcrystal preparations used when measuring the absorption spectra shown in Fig. 5(b) were not accurately described. Microcrystalline samples were reduced by the addition of dithiothreitol and mixed with a photocage (Sandelin et al., 2024 ▸), and the absorption spectrum for the reduced state was recorded. The sample was translated, a laser flash of 355 nm was used to release dioxygen, and an absorption spectrum for the oxidized state was recorded after a delay of 1 ms. The absorption contribution of the photocage was subtracted from both spectra. Spectra from detergent-solubilized samples of cytochrome c oxidase (CcO) [Fig. 5(a)] were prepared as described: the oxidized spectrum is recorded from CcO samples as isolated, the reduced spectrum is recorded after treatment with sodium di­thio­nite under anaerobic conditions, and no photocage was used in those measurements.