New Degenerate Cytophaga-Flexibacter-Bacteroides-Specific 16S Ribosomal DNA-Targeted Oligonucleotide Probes Reveal High Bacterial Diversity in River Taff Epilithon

Volume 68, no. 1, p. 201-210, 2002. Page 203, column 1, line 39: “for 2 h in 5× 0,” should read “for 2 h in 5× SSC-2% blocking solution (Boehringer Mannheim)-0.1% N-lauryl sarcosine-0.02% SDS. Hybridization was carried out for 12 to 18 h in hybridization buffer containing 0.9 M NaCl-0.1% N-lauryl sarcosine-4% blocking reagent-0.01% SDS-formamide (20, 30, 40, 50, and 60%)-20 ng of probe ml⁻¹. Stringency washes were carried out twice for 15 min in 0.01% SDS-0.02 M Tris-HCl (pH 7.4)-NaCl (0.19, 0.074, 0.037, 0.019, and 0.013 M). Optimal hybridization conditions were chosen when there was no detection for nontarget organisms and substantial detection for target CFBs. Optimal hybridization conditions for probes CFB560, CFB562, and CFB376 were found at 40.”