In the original publication [1], there was a mistake in Figure 3A. This data error occurred due to an unforeseen mistake during the data organization process. The corrected Figure 3 appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Figure 3.
Prednisolone (PDS)-induced anti-osteogenic activity was inhibited by pretreatment with FO in MC3T3-E1 cells. MC3T3-E1 cells (1 × 104 cells/mL) were pretreated with FO (0–200 μg/mL) for 2 h prior to treatment with 10 μM PDS for seven days. Fresh media with FO and/or PDS were replenished every two days. At day 7, (A) bone mineralization and (B) ALP activity were evaluated using alizarin red staining and a TRACP & ALP Double-Staining Kit, respectively. (C) Total mRNA was extracted, and RT-PCR was performed to evaluate the gene expressions of ALP, RUNX2, and OSX. GAPDH was used as the internal control. (D) Total proteins were extracted, and Western blotting was performed to evaluate the expression of ALP, RUNX2, and OSX. β-Actin was used as the internal control. All data are presented as means ± standard error of the mean (### p < 0.001 vs. untreated MC3T3-E1 cells; * p < 0.05, and *** p < 0.001 vs. PDS-treated MC3T3-E1 cells). ALP: alkaline phosphatase; RUNX2: runt-related transcription factor 2; and OSX: osterix.
Footnotes
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
Reference
- 1.Molagoda I.M.N., Athapaththu A.M.G.K., Park E.K., Choi Y.H., Jeon Y.-J., Kim G.-Y. Fermented Oyster (Crassostrea gigas) Extract Cures and Prevents Prednisolone-Induced Bone Resorption by Activating Osteoblast Differentiation. Foods. 2022;11:678. doi: 10.3390/foods11050678. [DOI] [PMC free article] [PubMed] [Google Scholar]

