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. 1966 Oct;101(1):214–228. doi: 10.1042/bj1010214

Location of disulphide bridges by diagonal paper electrophoresis

The disulphide bridges of bovine chymotrypsinogen A

J R Brown 1, B S Hartley 1
PMCID: PMC1270086  PMID: 5971783

Abstract

1. A new method is described for `fingerprinting' cysteic acid peptides derived from the disulphide bridges of proteins. Cystine peptides are separated by paper electrophoresis and oxidized on paper by performic acid vapour. Electrophoresis at right angles to the first direction produces parallel groups of cysteic acid peptides lying off a diagonal. This `fingerprint' reveals the way in which the cysteic acid peptides were originally joined in the protein. 2. The method allows a very easy selective purification of cysteic acid peptides. 3. By applying this method to bovine chymotrypsinogen A, we found that the half-cystine residues were linked 1–122, 42–58, 136–201, 168–182 and 191–220.

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Selected References

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