Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1966 Nov;101(2):385–391. doi: 10.1042/bj1010385

The further preparation of inorganic cationic yeasts and some of their chief properties

E J Conway 1, Helen M Gaffney 1
PMCID: PMC1270118  PMID: 5966275

Abstract

1. A method is described for replacing the intracellular K+ of the yeast cell by Rb+, Cs+, Li+ or Ca2+ ions. In the formation of a calcium yeast it is necessary to proceed first through a sodium yeast (Conway & Moore, 1954) as in the formation of a magnesium yeast (Conway & Beary, 1962). This concludes the series of such yeasts in which almost all the usual K+ is replaced by another cation, and for which the effect on the properties of fermentation, oxygen uptake and of growth are described. 2. Previous work has shown that all these inorganic cations that can be accumulated in quantity at pH7·0 are taken up by the same carrier, that the uptake of Mg2+ is almost completely inhibited by anoxia and cyanide (0·2mm) and that in the uptake of Mg2+ ions a practically equivalent amount of H+ ions is excreted. It is suggested that these facts amount to a definitive demonstration that the carrier is a cytochrome.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CONWAY E. J., ARMSTRONG W. M. The total intracellular concentration of solutes in yeast and other plant cells and the distensibility of the plant-cell wall. Biochem J. 1961 Dec;81:631–639. doi: 10.1042/bj0810631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CONWAY E. J., BEARY M. E. A magnesium yeast and its properties. Biochem J. 1962 Aug;84:328–333. doi: 10.1042/bj0840328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CONWAY E. J., BEARY M. E. Active transport of magnesium across the yeast cell membrane. Biochem J. 1958 Jun;69(2):275–280. doi: 10.1042/bj0690275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CONWAY E. J., DOWNEY M. pH values of the yeast cell. Biochem J. 1950 Sep;47(3):355–360. doi: 10.1042/bj0470355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CONWAY E. J., DUGGAN F. A cation carrier in the yeast cell wall. Biochem J. 1958 Jun;69(2):265–274. doi: 10.1042/bj0690265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CONWAY E. J., MCCORMACK J. I. The total intracellular concentration of mammalian tissues compared with that of the extra-cellular fluid. J Physiol. 1953 Apr 28;120(1-2):1–14. doi: 10.1113/jphysiol.1953.sp004867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CONWAY E. J., MOORE P. T. A sodium-yeast and some of its properties. Biochem J. 1954 Jul;57(3):523–528. doi: 10.1042/bj0570523. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES