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. 1966 Nov;101(2):441–447. doi: 10.1042/bj1010441

The hydrating effect of lathyrogenic compounds on chick-embryo cartilage in vivo

C I Levene 1,*
PMCID: PMC1270126  PMID: 5966280

Abstract

1. Treatment of the chick embryo with the lathyrus factor, β-aminopropionitrile, produces inhibition of polymerization of newly synthesized collagen at the interand intra-molecular level (Levene & Gross, 1959; Martin, Gross, Piez & Lewis, 1961); this is manifest as an increase in the fragility and solubility of collagen from the connective tissues. 2. This treatment simultaneously produced increased hydration of the tissues, particularly noticeable in the swollen long-bone cartilages. 3. This increased hydration of cartilage was found to result from treatment with any of the known lathyrogenic compounds, but not with any of the structurally related but non-lathyrogenic analogues. 4. The hydration effect paralleled the collagen solubility effect, both being dosage-dependent at the lower levels. 5. The hydration effect could not be reversed by treatment in vivo with either pyridoxal or glyceraldehyde, or by Benadryl, an antihistamine that inhibits cell water movement. 6. The possible causes of this effect have been considered in the light of the known properties of cartilage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BORLE A. B., KARNOVSKY M. J., NICHOLS G., Jr Changes in inorganic composition of tissues in experimental osteolathyrism. Am J Physiol. 1959 Dec;197:1224–1228. doi: 10.1152/ajplegacy.1959.197.6.1224. [DOI] [PubMed] [Google Scholar]
  2. FESSLER J. H. A structural function of mucopolysaccharide in connective tissue. Biochem J. 1960 Jul;76:124–132. doi: 10.1042/bj0760124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GERSH I., CATCHPOLE H. R. The nature of ground substance of connective tissue. Perspect Biol Med. 1960;3:282–319. doi: 10.1353/pbm.1960.0019. [DOI] [PubMed] [Google Scholar]
  4. GROSS J., LEVENE C. I., ORLOFF S. Fragility and extractable collagen in the lathyritic chick embryo. An assay for lathyrogenic agents. Proc Soc Exp Biol Med. 1960 Oct;105:148–151. doi: 10.3181/00379727-105-26040. [DOI] [PubMed] [Google Scholar]
  5. GROSS J. Studies on the formation of collagen. II. The influence of growth rate on neutral salt extracts of guinea pig dermis. J Exp Med. 1958 Feb 1;107(2):265–277. doi: 10.1084/jem.107.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JUDAH J. D. Effect of antihistamines on mitochondrial swelling and liver injury. Nature. 1960 Feb 6;185:390–391. doi: 10.1038/185390a0. [DOI] [PubMed] [Google Scholar]
  7. KANTOR T. G., SCHUBERT M. The difference in permeability of cartilage to cationic and anionic dyes. J Histochem Cytochem. 1957 Jan;5(1):28–32. doi: 10.1177/5.1.28. [DOI] [PubMed] [Google Scholar]
  8. KUNDEL H. L. OSTEOLATHYRISM IN THE RAT; AN X-RAY DIFFRACTION STUDY OF COLLAGEN FROM NORMAL AND LATHYRITIC RATS. Radiology. 1964 Jan;82:67–76. doi: 10.1148/82.1.67. [DOI] [PubMed] [Google Scholar]
  9. LEVENE C. I., GROSS J. Alterations in state of molecular aggregation of collagen induced in chick embryos by beta-aminopropionitrile (lathyrus factor). J Exp Med. 1959 Nov 1;110:771–790. doi: 10.1084/jem.110.5.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LEVENE C. I. Structural requirements for lathyrogenic agents. J Exp Med. 1961 Sep 1;114:295–310. doi: 10.1084/jem.114.3.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LEVENE C. I. The lathyrogenic effect of isonicotinic acid hydrazide (INAH) on the chick embryo and its reversal by pyridoxal. J Exp Med. 1961 Apr 1;113:795–810. doi: 10.1084/jem.113.4.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LINN F. C., SOKOLOFF L. MOVEMENT AND COMPOSITION OF INTERSTITIAL FLUID OF CARTILAGE. Arthritis Rheum. 1965 Aug;8:481–494. doi: 10.1002/art.1780080402. [DOI] [PubMed] [Google Scholar]
  13. Levene C. I., Kranzler J., Franco-Browder S. Acid mucopolysaccharides of chick-embryo cartilage in osteolathyrism. Biochem J. 1966 Nov;101(2):435–440. doi: 10.1042/bj1010435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MARTIN G. R., GROSS J., PIEZ K. A., LEWIS M. S. On the intramolecular cross-linking of collagen in lathyritic rats. Biochim Biophys Acta. 1961 Nov 11;53:599–601. doi: 10.1016/0006-3002(61)90227-x. [DOI] [PubMed] [Google Scholar]
  15. MILES J. S., EICHELBERGER L. BIOCHEMICAL STUDIES OF HUMAN CARTILAGE DURING THE AGING PROCESS. J Am Geriatr Soc. 1964 Jan;12:1–20. doi: 10.1111/j.1532-5415.1964.tb01535.x. [DOI] [PubMed] [Google Scholar]
  16. Milch R. A. Hydrated density of chondroitin sulfate preparations. Experientia. 1965 Oct 15;21(10):578–579. doi: 10.1007/BF02151541. [DOI] [PubMed] [Google Scholar]
  17. NIKKARI T., KULONEN E. Studies in experimental lathyrism. II. On the properties of collagen. Biochem Pharmacol. 1962 Oct;11:931–936. doi: 10.1016/0006-2952(62)90142-9. [DOI] [PubMed] [Google Scholar]
  18. OGSTON A. G., PHELPS C. F. The partition of solutes between buffer solutions and solutions containing hyaluronic acid. Biochem J. 1961 Apr;78:827–833. doi: 10.1042/bj0780827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SCHRYVER H. F., BIGGERS J. D. THE EFFECT OF LATHYROGENIC AGENTS ON EMBRYONIC CHICK TIBIOTARSI IN VITRO. J Exp Zool. 1963 Dec;154:339–351. doi: 10.1002/jez.1401540308. [DOI] [PubMed] [Google Scholar]
  20. STAMLER F. W. Reproduction in rats fed Lathyrus peas or aminonitriles. Proc Soc Exp Biol Med. 1955 Oct;90(1):294–298. doi: 10.3181/00379727-90-22013. [DOI] [PubMed] [Google Scholar]

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