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. 1966 Dec;101(3):811–818. doi: 10.1042/bj1010811

Studies in lipogenesis in vivo

Fatty acid and cholesterol synthesis during starvation and re-feeding

G R Jansen 1, M E Zanetti 1, C F Hutchison 1
PMCID: PMC1270189  PMID: 16742461

Abstract

1. Lipogenesis in vivo has been studied in mice given a 250mg. meal of [U-14C]glucose (2·5μc) or given an intraperitoneal injection of 25μg. of [U-14C]glucose (2·0μc). 2. The ability to convert a [U-14C]glucose meal into fatty acid was not significantly depressed by 6–7hr. of starvation. In contrast, incorporation of 14C into fatty acid in the liver after the intraperitoneal dose of [14C]glucose was depressed by 80% and by more than 90% by 1 and 2hr. of starvation respectively. Carcass fatty acid synthesis from the [U-14C]glucose meal was not depressed by 12hr. of starvation, whereas from the tracer dose of [U-14C]glucose the depression in incorporation was 80% after 6hr. of starvation. 3. Re-feeding for 3 days, after 3 days' starvation, raised fatty acid synthesis and cholesterol synthesis in the liver fivefold and tenfold respectively above the levels in non-starved control mice. These increases were associated with an increased amount of both fatty acid and cholesterol in the liver. 4. After 18hr. of starvation incorporation of a [U-14C]glucose meal into carcass and liver glycogen were both increased threefold.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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