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. 1967 Feb;102(2):606–611. doi: 10.1042/bj1020606

Formation of retinoic acid from retinol in the rat

R J Emerick 1,*, Maija Zile 1, H F DeLuca 1
PMCID: PMC1270286  PMID: 6029617

Abstract

1. The formation in vivo of retinoic acid from microgram quantities of intrajugularly administered [15-14C]retinol was demonstrated in the rat. 2. Endogenously formed retinoic acid (about 0·1μg./rat) was found in liver, and to a much smaller extent in intestine, 12hr. after retinol administration. 3. Excretion of some of the endogenously formed retinoic acid occurred in the bile of bile-duct-cannulated rats. 4. Excretion of unaltered retinoic acid in the urine of intact rats did not occur even after the intrajugular administration of preformed retinoic acid.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. DELUCA H. F., MANATT M. R., MADSEN N., OLSON E. B. ACTION OF VITAMIN A ON LIVER HOMOGENATE OXIDATION OF TRICARBOXYLIC ACID CYCLE INTERMEDIATES. J Nutr. 1963 Dec;81:383–386. doi: 10.1093/jn/81.4.383. [DOI] [PubMed] [Google Scholar]
  3. DUNAGIN P. E., Jr, ZACHMAN R. D., OLSON J. A. IDENTIFICATION OF FREE AND CONJUGATED RETINOIC ACID AS A PRODUCT OF RETINAL (VITAMIN A ALDEHYDE) METABOLISM IN THE RAT IN VIVO. Biochim Biophys Acta. 1964 Aug 19;90:432–434. doi: 10.1016/0304-4165(64)90218-1. [DOI] [PubMed] [Google Scholar]
  4. Deshmukh D. S., Malathi P., Ganguly J. Rapid conversion of retinal (vitamin A aldehyde) to retinoic acid (vitamin A acid) in the living rat. Biochim Biophys Acta. 1965 Aug 24;107(1):120–122. doi: 10.1016/0304-4165(65)90394-6. [DOI] [PubMed] [Google Scholar]
  5. Dowling J. E., Wald G. THE BIOLOGICAL FUNCTION OF VITAMIN A ACID. Proc Natl Acad Sci U S A. 1960 May;46(5):587–608. doi: 10.1073/pnas.46.5.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FISCHER G. A., KABARA J. J. SIMPLE, MULTIBORE COLUMNS FOR SUPERIOR FRACTIONATION OF LIPIDS. Anal Biochem. 1964 Nov;9:303–309. doi: 10.1016/0003-2697(64)90188-5. [DOI] [PubMed] [Google Scholar]
  7. HARRIS P. L. Bioassay of vitamin A compounds. Vitam Horm. 1960;18:341–370. doi: 10.1016/s0083-6729(08)60868-7. [DOI] [PubMed] [Google Scholar]
  8. HOWELL J. M., THOMPSON J. N., PITT G. A. HISTOLOGY OF THE LESIONS PRODUCED IN THE REPRODUCTIVE TRACT OF ANIMALS FED A DIET DEFICIENT IN VITAMIN A ALCOHOL BUT CONTAINING VITAMIN A ACID. II. THE FEMALE RAT. J Reprod Fertil. 1964 Apr;7:251–258. doi: 10.1530/jrf.0.0070251. [DOI] [PubMed] [Google Scholar]
  9. JURKOWITZ L. Determination of vitamin A acid in human plasma after oral administration. Arch Biochem Biophys. 1962 Aug;98:337–341. doi: 10.1016/0003-9861(62)90192-3. [DOI] [PubMed] [Google Scholar]
  10. KRISHNAMURTHY S., BIERI J. G., ANDREWS E. L. Metabolism and biological activity of vitamin A acid in the chick. J Nutr. 1963 Apr;79:503–510. doi: 10.1093/jn/79.4.503. [DOI] [PubMed] [Google Scholar]
  11. REDFEARN E. R. The metabolism of vitamin A acid and its C25 homolog. Arch Biochem Biophys. 1960 Dec;91:226–229. doi: 10.1016/0003-9861(60)90494-x. [DOI] [PubMed] [Google Scholar]
  12. ROSENBERG H., ENNOR A. H., SUGAI S. BIOSYNTHESIS OF L-SERINE ETHANOLAMINE PHOSPHODIESTER. Nature. 1964 Jul 25;203:410–410. doi: 10.1038/203410a0. [DOI] [PubMed] [Google Scholar]
  13. Roberts A. B., DeLuca H. F. Pathways of retinol and retinoic acid metabolism in the rat. Biochem J. 1967 Feb;102(2):600–605. doi: 10.1042/bj1020600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. THOMPSON J. N., HOWELL J. M., PITT G. A. VITAMIN A AND REPRODUCTION IN RATS. Proc R Soc Lond B Biol Sci. 1964 Feb 18;159:510–535. doi: 10.1098/rspb.1964.0017. [DOI] [PubMed] [Google Scholar]
  15. Zile M., Deluca H. F. A biologically active metabolite of retinoic acid from rat liver. Biochem J. 1965 Oct;97(1):180–186. doi: 10.1042/bj0970180. [DOI] [PMC free article] [PubMed] [Google Scholar]

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