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. 1967 Mar;102(3):870–877. doi: 10.1042/bj1020870

Studies on lipogenesis in vivo

Fatty acid and cholesterol synthesis in hyperglycaemic–obese mice

G R Jansen 1, M E Zanetti 1, C F Hutchison 1
PMCID: PMC1270338  PMID: 16742504

Abstract

1. Lipogenesis has been studied in intact genetically obese mice by measuring the incorporation of a single oral dose of 250mg. of [U-14C]glucose into fatty acid and cholesterol in the liver and extrahepatic tissues. Studies were also carried out with [U-14C]glucose added to the diet and fed for 24hr. With either method of isotope administration, the conversion of [U-14C]glucose into fatty acid was greatly elevated in the livers of the obese mice. In contrast, conversion of the single dose of [14C]glucose into fatty acid in extrahepatic tissues of obese mice was only half that occurring in the non-obese litter mates. When [14C]glucose was given in the diet for 24hr. the total accumulation of labelled fatty acid in extrahepatic tissues of obese mice was slightly less than in the non-obese. Uptake of labelled glucose and conversion into fatty acid in adipose tissue of the obese mice decreased with age. 2. Conversion of the single dose of [14C]glucose into liver cholesterol was comparable in obese and non-obese mice fed on a purified low-fat diet. However, obese mice given this diet for 12 weeks accumulated 1·54% of cholesterol in the liver compared with 0·29% in the non-obese litter mates. This accumulation apparently resulted from a decrease in removal of cholesterol from the liver, rather than an increased synthesis. 3. Conversion of the single dose of [14C]glucose into extrahepatic fatty acid was decreased by 18hr. starvation proportionally as much in obese as in non-obese mice. The decrease in liver fatty acid synthesis caused by starvation also was considerable in obese mice, although somewhat less marked than in the non-obese. 4. The metabolic derangements in the liver could be more fundamental to the development of the obesity than the changes seen in extrahepatic tissues.

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Selected References

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