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. 1967 Apr;103(1):230–237. doi: 10.1042/bj1030230

The degradation of ribonucleic acid in the cotyledons of Pisum arvense

G R Barker 1, José A Hollinshead 1,*
PMCID: PMC1270390  PMID: 6033762

Abstract

1. A ribonuclease has been partially purified from the cotyledons of germinating seed of Pisum arvense. 2. The enzyme degrades ribopolynucleotides to adenosine 3′-phosphate, guanosine 3′-phosphate and the cyclic nucleotides cytidine 2′,3′-phosphate and uridine 2′,3′-phosphate; no resistant `core' remains. 3. The activity of RNA-degrading enzymes in the cotyledons increases to a maximum during the first 5 days of germination, passes through a minimum around the eighth day, and thereafter increases again. 4. Ion-exchange chromatography of methanol-soluble extracts of cotyledons revealed the presence, amongst other components, of the 2′-, 3′- and 5′-phosphates of cytidine and uridine, the 3′- and 5′-phosphates of adenosine, and guanosine 5′-phosphate. 5. Seed soaked in a solution containing [32P]orthophosphate gave a methanol-soluble fraction containing labelled nucleoside 5′-phosphates, but nucleoside 2′- and 3′-phosphates were not labelled. 6. It is believed that the nucleoside 2′- and 3′-phosphates arise by the action of ribonuclease on cotyledon RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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