Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 May;103(2):331–341. doi: 10.1042/bj1030331

Measurement of growth hormone released by ox anterior-pituitary slices in vitro

J G Schofield 1
PMCID: PMC1270413  PMID: 4962082

Abstract

1. A method has been developed for the iodination of ox growth hormone by using iodine monochloride, giving a product which is 93±4% bindable to antiserum. 2. The 131I- or 125I-labelled growth hormone obtained has been used in the development of an immunological assay for growth hormone. 3. The assay could be applied to the measurement of growth-hormone output by pituitary slices in vitro. 4. On incubation ox-pituitary slices liberate 8·1% of their growth-hormone content in the first hour and 4·5% in the second hour. 5. The amount of growth hormone liberated into the medium is greater if the tissue is frequently transferred than if it is incubated in the same medium. 6. The output is stimulated by a hypothalamic extract but inhibited by high concentrations of posterior-lobe powder: a stalk–median-eminence extract had no effect. 7. The output is lowered by temperature reduction, β-hydroxybutyrate, acetoacetate, and pyruvate, glutamate and fumarate. Palmitoylcarnitine stimulates growth-hormone output. 8. Anoxia and 2,4-dinitrophenol have no effect on output.

Full text

PDF
331

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDO S., GUZE L. B., GOLD E. M. ACTH RELEASE IN VIVO AND IN VITRO: EXTRAPITUITARY MEDIATION DURING ESCH. COLI BACTEREMIA. Endocrinology. 1964 Jun;74:894–901. doi: 10.1210/endo-74-6-894. [DOI] [PubMed] [Google Scholar]
  2. BROWN J. B., BULBROOK R. D., GREENWOOD F. C. An evaluation of a chemical method for the estimation of oestriol, oestrone and oestradiol-17 beta in human urine. J Endocrinol. 1957 Nov;16(1):41–48. doi: 10.1677/joe.0.0160041. [DOI] [PubMed] [Google Scholar]
  3. Cerasi E., Della Casa L., Luft R., Roovete A. Determination of human growth hormone (HGH) in plasma by a double antibody radioimmunoassay. Acta Endocrinol (Copenh) 1966 Sep;53(1):101–120. doi: 10.1530/acta.0.0530101. [DOI] [PubMed] [Google Scholar]
  4. Coore H. G., Randle P. J. Inhibition of glucose phosphorylation by mannoheptulose. Biochem J. 1964 Apr;91(1):56–59. doi: 10.1042/bj0910056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DELLACHA J. M., SONENBERG M. PURIFICATION OF BOVINE GROWTH HORMONE. J Biol Chem. 1964 May;239:1515–1520. [PubMed] [Google Scholar]
  6. FERGUSON K. A., WALLACE A. L. Starch-gel electrophoresis of anterior pituitary hormones. Nature. 1961 May 13;190:629–630. doi: 10.1038/190629a0. [DOI] [PubMed] [Google Scholar]
  7. FRANZ J., HASELBACH C. H., LIBERT O. Studies of the effect of hypothalamic extracts on somatotrophic pituitary function. Acta Endocrinol (Copenh) 1962 Nov;41:336–350. doi: 10.1530/acta.0.0410336. [DOI] [PubMed] [Google Scholar]
  8. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HALES C. N., RANDLE P. J. Immunoassay of insulin with insulin-antibody precipitate. Biochem J. 1963 Jul;88:137–146. doi: 10.1042/bj0880137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HAYASHIDA T., GRUNBAUM B. W. Demonstration of several components in highly purified human growth hormone by immunodiffusion on cellulose acetate. Endocrinology. 1962 Nov;71:734–739. doi: 10.1210/endo-71-5-734. [DOI] [PubMed] [Google Scholar]
  11. HUNTER W. M., GREENWOOD F. C. STUDIES ON THE SECRETION OF HUMAN-PITUITARY-GROWTH HORMONE. Br Med J. 1964 Mar 28;1(5386):804–807. doi: 10.1136/bmj.1.5386.804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MORGAN C. R., SORENSON R. L., LAZAROW A. FURTHER STUDIES OF AN INHIBITOR OF THE TWO ANTIBODY IMMUNOASSAY SYSTEM. Diabetes. 1964 Nov-Dec;13:579–584. doi: 10.2337/diab.13.6.579. [DOI] [PubMed] [Google Scholar]
  13. McFarlane A. S. IN VIVO BEHAVIOR OF I-FIBRINOGEN. J Clin Invest. 1963 Mar;42(3):346–361. doi: 10.1172/JCI104721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHALLY A. V., STEELMAN S. L., BOWERS C. Y. EFFECT OF HYPOTHALAMIC EXTRACTS ON RELEASE OF GROWTH HORMONE IN VITRO. Proc Soc Exp Biol Med. 1965 May;119:208–212. doi: 10.3181/00379727-119-30138. [DOI] [PubMed] [Google Scholar]
  15. Solomon S. H., McKenzie J. M. Release of thyrotropin by the rat pituitary gland in vitro. Endocrinology. 1966 Apr;78(4):699–706. doi: 10.1210/endo-78-4-699. [DOI] [PubMed] [Google Scholar]
  16. UTIGER R. D., PARKER M. L., DAUGHADAY W. H. Studies on human growth hormone. I. A radio-immunoassay for human growth hormone. J Clin Invest. 1962 Feb;41:254–261. doi: 10.1172/JCI104478. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES