Abstract
1. When Escherichia coli was grown exponentially in a defined medium at 35°, the rate of protein breakdown was initially rapid, but decreased to 0·6%/hr. after about 30min. The latter rate was maintained for at least 3·5hr. 2. The initial rapid rate may have been due to the presence of a small protein fraction (about 1%) that was degraded with a half-life of 13min. 3. The rate of protein degradation was the same during balanced growth at low rates imposed in a bactogen. However, it increased during the period immediately after a decrease of the growth rate.
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Selected References
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- COHEN G. N., RICKENBERG H. V. Concentration spécifique réversible des amino acides chez Escherichia coli. Ann Inst Pasteur (Paris) 1956 Nov;91(5):693–720. [PubMed] [Google Scholar]
- COWIE D. B., BOLTON E. T., SANDS M. K. Sulfur metabolism in Escherichia coli. I. Sulfate metabolism of normal and mutant cells. J Bacteriol. 1950 Sep;60(3):233–248. doi: 10.1128/jb.60.3.233-248.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOCH A. L. Death of bacteria in growing culture. J Bacteriol. 1959 May;77(5):623–629. doi: 10.1128/jb.77.5.623-629.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOCH A. L., LEVY H. R. Protein turnover in growing cultures of Escherichia coli. J Biol Chem. 1955 Dec;217(2):947–957. [PubMed] [Google Scholar]
- MANDELSTAM J. Turnover of protein in growing and non-growing populations of Escherichia coli. Biochem J. 1958 May;69(1):110–119. doi: 10.1042/bj0690110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pine M. J. Heterogeneity of protein turnover in Escherichia coli. Biochim Biophys Acta. 1965 Jul 8;104(2):439–456. doi: 10.1016/0304-4165(65)90349-1. [DOI] [PubMed] [Google Scholar]
- ROGERS H. J. The preferential suppression of hyaluronidase formation in cultures of Staphylococcus aureus. J Gen Microbiol. 1957 Feb;16(1):22–37. doi: 10.1099/00221287-16-1-22. [DOI] [PubMed] [Google Scholar]
- Willetts N. S. Intracellular protein breakdown in non-growing cells of Escherichia coli. Biochem J. 1967 May;103(2):453–461. doi: 10.1042/bj1030453. [DOI] [PMC free article] [PubMed] [Google Scholar]