Abstract
Highly purified chromaffin granules can be obtained from homogenates of either ox, pig, horse or rat adrenal medullae by ultracentrifugation of the large-granule fraction layered on 1·6m-sucrose solution, by using angle-head rotors. The chromaffin granules are obtained as a pink sediment that is only slightly contaminated by mitochondria and lysosomes.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANKS P. THE ADENOSINE-TRIPHOSPHATASE ACTIVITY OF ADRENAL CHROMAFFIN GRANULES. Biochem J. 1965 May;95:490–496. doi: 10.1042/bj0950490. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BLASCHKO H., BORN G. V., D'IORIO A., EADE N. R. Observations on the distribution of catechol amines and adenosinetriphosphate in the bovine adrenal medulla. J Physiol. 1956 Sep 27;133(3):548–557. doi: 10.1113/jphysiol.1956.sp005607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BLASCHKO H., HAGEN J. M., HAGEN P. Mitochondrial enzymes and chromaffin granules. J Physiol. 1957 Dec 3;139(2):316–322. doi: 10.1113/jphysiol.1957.sp005893. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EADE N. R. The distribution of the catechol amines in homogenates of the bovine adrenal medulla. J Physiol. 1958 Apr 3;141(1):183–192. doi: 10.1113/jphysiol.1958.sp005966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ichikawa Y., Yamano T. Cytochrome 559 in the microsomes of the adrenal medulla. Biochem Biophys Res Commun. 1965 Jul 26;20(3):263–268. doi: 10.1016/0006-291x(65)90357-8. [DOI] [PubMed] [Google Scholar]
- RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
- STREHLER B. L., TOTTER J. R. Determination of ATP and related compounds: firefly luminescence and other methods. Methods Biochem Anal. 1954;1:341–356. doi: 10.1002/9780470110171.ch13. [DOI] [PubMed] [Google Scholar]
- Smith A. D., Winkler H. Purification and properties of an acidic protein from chromaffin granules of bovine adrenal medulla. Biochem J. 1967 May;103(2):483–492. doi: 10.1042/bj1030483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith A. D., Winkler H. The localization of lysosomal enzymes in chromaffin tissue. J Physiol. 1966 Mar;183(1):179–188. doi: 10.1113/jphysiol.1966.sp007859. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von EULER U., LISHAJKO F. Improved technique for the fluorimetric estimation of catecholamines. Acta Physiol Scand. 1961 Apr;51:348–355. doi: 10.1111/j.1748-1716.1961.tb02128.x. [DOI] [PubMed] [Google Scholar]