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. 1967 Jun;103(3):768–772. doi: 10.1042/bj1030768

The gas–liquid chromatography of carboxylic acid esters of the urinary 11-deoxy-17-oxo steroids

Determination as n-butyrates

Patricia A Sadler 1,*, A E Kellie 1
PMCID: PMC1270481  PMID: 4227802

Abstract

1. The gas–liquid-chromatographic separations of the acetate, propionate, n-butyrate, isobutyrate and n-valerate esters of androsterone, aetiocholanolone and dehydroepiandrosterone were studied on a 1% neopentyl glycol sebacate column. The n-butyrate, isobutyrate and n-valerate esters were well resolved. 2. The three steroids derived from hydrolysed urinary 17-oxo steroid conjugate extracts were analysed by gas–liquid chromatography after conversion into their n-butyrate esters. The results were compared with independent determinations involving chromatography on alumina.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURSTEIN S., LIEBERMAN S. Hydrolysis of ketosteroid hydrogen sulfates by solvolysis procedures. J Biol Chem. 1958 Aug;233(2):331–335. [PubMed] [Google Scholar]
  2. BUSH I. E. Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues. Biochem J. 1952 Jan;50(3):370–378. doi: 10.1042/bj0500370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Callow N. H., Callow R. K., Emmens C. W. Colorimetric determination of substances containing the grouping -CH(2).CO- in urine extracts as an indication of androgen content. Biochem J. 1938 Aug;32(8):1312–1331. doi: 10.1042/bj0321312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FRANCE J. T., RIVERA R., MCNIVEN N. L., DORFMAN R. I. DETERMINATION OF ANDROSTERONE, ETIOCHOLANOLONE AND DEHYDROEPIANDROSTERONE IN URINE BY GAS-LIQUID CHROMATOGRAPHY. Steroids. 1965 May;10:687–697. doi: 10.1016/0039-128x(65)90161-3. [DOI] [PubMed] [Google Scholar]
  5. HAAHTI E. O., VANDENHEUVEL W. J., HORNING E. C. Separation of urinary 17-ketosteroids by gas chromatography. Anal Biochem. 1961 Apr;2:182–187. doi: 10.1016/0003-2697(61)90070-7. [DOI] [PubMed] [Google Scholar]
  6. KELLIE A. E., WADE A. P. The analysis of urinary 17-oxo steroids by gradient elution. Biochem J. 1957 Jun;66(2):196–206. doi: 10.1042/bj0660196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KNIGHTS B. A., THOMAS G. H. Rm values in the gas-liquid chromatography of steroids. Nature. 1962 Jun 2;194:833–835. doi: 10.1038/194833a0. [DOI] [PubMed] [Google Scholar]
  8. VANDENHEUVEL W. J., CREECH B. G., HORNING E. C. Separation and estimation of the principal human urinary 17-ketosteroids as trimethylsilyl ethers. Anal Biochem. 1962 Sep;4:191–197. doi: 10.1016/0003-2697(62)90001-5. [DOI] [PubMed] [Google Scholar]

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