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. 1967 Jun;103(3):791–795. doi: 10.1042/bj1030791

[35S]Thiosulphate oxidation by rat liver mitochondria in the presence of glutathione

A Koj 1, J Frendo 1, Z Janik 1
PMCID: PMC1270484  PMID: 6049402

Abstract

1. Rat liver mitochondria incubated in oxygen with glutathione and [35S]-thiosulphate produced labelled sulphate. 2. Inner-labelled thiosulphate (S·35SO3)2− was converted into [35S]sulphate more rapidly than outer-labelled thiosulphate (35S·SO3)2−. 3. Thiosulphate labelled in both sulphur atoms was formed during (35S·SO3)2− oxidation; the outer sulphur atom before oxidation to sulphate was incorporated into the inner position. 4. A thiosulphate cycle in the metabolic pathway of sulphate formation in animal tissues is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAXTER C. F., VAN REEN R., PEARSON P. B., ROSENBERG C. Sulfide oxidation in rat tissues. Biochim Biophys Acta. 1958 Mar;27(3):584–591. doi: 10.1016/0006-3002(58)90390-1. [DOI] [PubMed] [Google Scholar]
  2. BAXTER C. F., VAN REEN R. Some aspects of sulfide oxidation by rat-liver preparations. Biochim Biophys Acta. 1958 Jun;28(3):567–573. doi: 10.1016/0006-3002(58)90520-1. [DOI] [PubMed] [Google Scholar]
  3. DE MARCO C., COLETTA M., CAVALLINI D. The transulfuration of sulfinates by polysulfides. Experientia. 1962 Mar 15;18:117–118. doi: 10.1007/BF02153846. [DOI] [PubMed] [Google Scholar]
  4. DE MARCO C., COLETTA M. Thiosulfate production during transamination of alaninethiosulfonic acid. Biochim Biophys Acta. 1961 Feb 18;47:257–261. doi: 10.1016/0006-3002(61)90284-0. [DOI] [PubMed] [Google Scholar]
  5. KOJ A., FRENDO J. The activity of cysteine desulphhydrase and rhodanase in animal tissues. Acta Biochim Pol. 1962;9:373–379. [PubMed] [Google Scholar]
  6. Kelly D. P., Syrett P. J. [35S]thiosulphate oxidation by Thiobacillus strain C. Biochem J. 1966 Feb;98(2):537–545. doi: 10.1042/bj0980537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Pirie N. W. The oxidation of thiosulphate to sulphate by tissue slices in vitro. Biochem J. 1934;28(3):1063–1075. doi: 10.1042/bj0281063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SINGER T. P., KEARNEY E. B. Intermediary metabolism of L-cysteinesulfinic acid in animal tissues. Arch Biochem Biophys. 1956 Apr;61(2):397–409. doi: 10.1016/0003-9861(56)90363-0. [DOI] [PubMed] [Google Scholar]
  10. SKARZYNSKI B., SZCZEPKOWSKI T. W., WEBER M. Thiosulphate metabolism in the animal organism. Nature. 1959 Sep 26;184(Suppl 13):994–995. doi: 10.1038/184994a0. [DOI] [PubMed] [Google Scholar]
  11. SORBO B. A colorimetric method for the determination of thiosulfate. Biochim Biophys Acta. 1957 Feb;23(2):412–416. doi: 10.1016/0006-3002(57)90346-3. [DOI] [PubMed] [Google Scholar]
  12. SORBO B. Enzymic transfer of sulfur from mercaptopyruvate to sulfate or sulfinates. Biochim Biophys Acta. 1957 May;24(2):324–329. doi: 10.1016/0006-3002(57)90201-9. [DOI] [PubMed] [Google Scholar]
  13. SORBO B. On the formation of thiosulfate from inorganic sulfide by liver tissue and heme compounds. Biochim Biophys Acta. 1958 Feb;27(2):324–329. doi: 10.1016/0006-3002(58)90339-1. [DOI] [PubMed] [Google Scholar]
  14. SORBO B. On the mechanism of sulfide oxidation in biological systems. Biochim Biophys Acta. 1960 Feb 26;38:349–351. doi: 10.1016/0006-3002(60)91255-5. [DOI] [PubMed] [Google Scholar]
  15. SPENCER B. The ultramicro determination of inorganic sulphte. Biochem J. 1960 Jun;75:435–440. doi: 10.1042/bj0750435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SZCZEPKOWSKI T. W. [Role of rhodanase in metabolic production of thiosulfates]. Acta Biochim Pol. 1961;8:251–264. [PubMed] [Google Scholar]
  17. TRUDINGER P. A. Thiosulphate oxidation and cytochromes in Thiobacillus X. 2. Thiosulphate-oxidizing enzyme. Biochem J. 1961 Apr;78:680–686. doi: 10.1042/bj0780680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. VILLAREJO M., WESTLEY J. MECHANISM OF RHODANESE CATALYSIS OF THIOSULFATE-LIPOATE OXIDATION-REDUCTION. J Biol Chem. 1963 Dec;238:4016–4020. [PubMed] [Google Scholar]
  19. WEINBACH E. C. A procedure for isolating stable mitochondria from rat liver and kidney. Anal Biochem. 1961 Aug;2:335–343. doi: 10.1016/0003-2697(61)90006-9. [DOI] [PubMed] [Google Scholar]

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