Abstract
1. The distribution pattern of hyaluronidase in subcellular fractions of bone-tissue homogenates is closely similar to that reported by Vaes & Jacques (1965b) for the other acid hydrolases of this tissue. The highest specific activity of hyaluronidase is also found in the light-mitochondrial fraction. 2. In cytoplasmic extracts of bone, about 60% of the activity of hyaluronidase is latent, and is unmasked by a number of treatments (digitonin, low osmotic pressure, freezing and thawing, Waring Blendor) that unmask the lysosomal β-glucuronidase in a closely parallel manner. Low concentrations of Triton X-100 render a larger proportion of β-glucuronidase than of hyaluronidase accessible to external substrates, but release the same proportion of both enzymes in unsedimentable form. 3. These results support the concept of an association of hyaluronidase with lysosomes in bone.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOLLET A. J., BONNER W. M., Jr, NANCE J. L. THE PRESENCE OF HYALURONIDASE IN VARIOUS MAMMALIAN TISSUES. J Biol Chem. 1963 Nov;238:3522–3527. [PubMed] [Google Scholar]
- DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutterer F. Degradation of mucopolysaccharides by hepatic lysosomes. Biochim Biophys Acta. 1966 Feb 28;115(2):312–319. doi: 10.1016/0304-4165(66)90430-2. [DOI] [PubMed] [Google Scholar]
- Vaes G., Jacques P. Studies on bone enzymes. Distribution of acid hydrolases, alkaline phenylphosphatase, cytochrome oxidase and catalase in subcellular fraction of bone tissue homogenates. Biochem J. 1965 Nov;97(2):389–392. doi: 10.1042/bj0970389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaes G., Jacques P. Studies on bone enzymes. The assay of acid hydrolases and other enzymes in bone tissue. Biochem J. 1965 Nov;97(2):380–388. doi: 10.1042/bj0970380. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaes G. Studies on bone enzymes. The activation and release of latent acid hydrolases and catalase in bone-tissue homogenates. Biochem J. 1965 Nov;97(2):393–402. doi: 10.1042/bj0970393. [DOI] [PMC free article] [PubMed] [Google Scholar]