Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Jul;104(1):71–77. doi: 10.1042/bj1040071

The effect of osmotic `shock' on the swelling pattern and respiratory control of rat-liver mitochondria

Sara Streichman 1, Y Avi-Dor 1
PMCID: PMC1270546  PMID: 6035525

Abstract

1. Rat-liver mitochondria suspended in 0·25m-sucrose were exposed for a few seconds to strongly hypo-osmotic conditions, and then the osmolarity of the medium was raised again to 0·25 with the aid of tris chloride (osmotic `shock'). 2. Mitochondria after hypo-osmotic pretreatment lost their capacity for slow energy-dependent swelling in iso-osmotic tris buffer and showed no respiratory control. 3. Swelling could be induced in the `shocked' mitochondria by ATP but not by addition of respiratory substrates. 4. It was shown that cytochrome c is lost from `shocked' mitochondria when they come into contact with the tris buffer present in the assay medium, and that the changes observed in the pattern of swelling, as well as in respiratory control, are directly connected with this loss of cytochrome c. 5. The results of the investigation are discussed with regard to the role of cytochrome c in swelling and respiratory control.

Full text

PDF
71

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVI-DOR Y., LAMDIN E., KAPLAN N. O. Structural factors in the succinate-induced reduction of mitochondrial pyridine nucleotides. J Biol Chem. 1963 Jul;238:2518–2528. [PubMed] [Google Scholar]
  2. Azzone G. F., Azzi A. Volume changes in liver mitochondria. Proc Natl Acad Sci U S A. 1965 May;53(5):1084–1089. doi: 10.1073/pnas.53.5.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHANCE B., WILLIAMS G. R. The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem. 1956;17:65–134. doi: 10.1002/9780470122624.ch2. [DOI] [PubMed] [Google Scholar]
  4. CHAPPELL J. B., GREVILLE G. D. Dependence of mitochondrial swelling on oxidizable substrates. Nature. 1958 Sep 20;182(4638):813–814. doi: 10.1038/182813a0. [DOI] [PubMed] [Google Scholar]
  5. CONNELLY J. L., LARDY H. A. THE EFFECT OF ADENOSINE TRIPHOSPHATE AND SUBSTRATE ON ORTHOPHOSPHATE-INDUCED MITOCHONDRIAL SWELLING AT ACID PH. J Biol Chem. 1964 Sep;239:3065–3070. [PubMed] [Google Scholar]
  6. GLICK J. L., BRONK J. R. THE INFLUENCE OF THYROID HORMONES ON NORMAL RAT-LIVER MITOCHONDRIA. II. THYROXINE EFFECTS ON PRETREATED MITOCHONDRIA. Biochim Biophys Acta. 1965 Jan 4;97:16–22. doi: 10.1016/0304-4165(65)90264-3. [DOI] [PubMed] [Google Scholar]
  7. HOFFSTEN P. E., HUNTER F. E., Jr, GEBICKI J. M., WEINSTEIN J. Formation of "lipid peroxide" under conditions which lead to swelling and lysis of rat liver mitochondria. Biochem Biophys Res Commun. 1962 May 4;7:276–280. doi: 10.1016/0006-291x(62)90190-0. [DOI] [PubMed] [Google Scholar]
  8. HUNTER F. E., Jr, GEBICKI J. M., HOFFSTEN P. E., WEINSTEIN J., SCOTT A. Swelling and lysis of rat liver mitochondria induced by ferrous ions. J Biol Chem. 1963 Feb;238:828–835. [PubMed] [Google Scholar]
  9. HUNTER F. E., Jr, SCOTT A., HOFFSTEN P. E., GEBICKI J. M., WEINSTEIN J., SCHNEIDER A. STUDIES ON THE MECHANISM OF SWELLING, LYSIS, AND DISTINTEGRATION OF ISOLATED LIVER MITOCHONDRIA EXPOSED TO MIXTURES OF OXIDIZED AND REDUCED GLUTATHIONE. J Biol Chem. 1964 Feb;239:614–621. [PubMed] [Google Scholar]
  10. HUNTER F. E., Jr, SCOTT A., WEINSTEIN J., SCHNEIDER A. EFFECTS OF PHOSPHATE, ARSENATE, AND OTHER SUBSTANCES ON SWELLING AND LIPID PEROXIDE FORMATION WHEN MITOCHONDRIA ARE TREATED WITH OXIDIZED AND REDUCED GLUTATHIONE. J Biol Chem. 1964 Feb;239:622–630. [PubMed] [Google Scholar]
  11. JACOBS E. E., SANADI D. R. The reversible removal of cytochrome c from mitochondria. J Biol Chem. 1960 Feb;235:531–534. [PubMed] [Google Scholar]
  12. LARDY H. A., CONNELLY J. L., JOHNSON D. ANTIBIOTIC STUDIES. II. INHIBITION OF PHOSPHORYL TRANSFER IN MITOCHONDRIA BY OLIGOMYCIN AND AUROVERTIN. Biochemistry. 1964 Dec;3:1961–1968. doi: 10.1021/bi00900a030. [DOI] [PubMed] [Google Scholar]
  13. LEHNINGER A. L. Phosphorylation coupled to oxidation of dihydrodiphosphopyridine nucleotide. J Biol Chem. 1951 May;190(1):345–359. [PubMed] [Google Scholar]
  14. LEHNINGER A. L. Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation. Physiol Rev. 1962 Jul;42:467–517. doi: 10.1152/physrev.1962.42.3.467. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. MCKNIGHT R. C., HUNTER F. E., Jr, OEHLERT W. H. MITOCHONDRIAL MEMBRANE GHOSTS PRODUCED BY LIPID PEROXIDATION INDUCED BY FERROUS ION. I. PRODUCTION AND GENERAL MORPHOLOGY. J Biol Chem. 1965 Aug;240:3439–3445. [PubMed] [Google Scholar]
  17. McKnight R. C., Hunter F. E., Jr Mitochondrial membrane ghosts produced by lipid peroxidation induced by ferrous ion. II. Composition and enzymatic activity. J Biol Chem. 1966 Jun 25;241(12):2757–2765. [PubMed] [Google Scholar]
  18. Penniston J. T., Vande Zande H., Green D. E. Mitochondrial particles resolved for ion translocation. II. Extraction of cofactors and restoration of activity. Arch Biochem Biophys. 1966 Mar;113(3):512–518. doi: 10.1016/0003-9861(66)90227-x. [DOI] [PubMed] [Google Scholar]
  19. Suranyi E. M., Avi-Dor Y. Swelling-contraction of mitochondria in hypotonic medium. Biochim Biophys Acta. 1966 Jun 15;118(3):445–452. doi: 10.1016/s0926-6593(66)80088-7. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES