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. 1967 Jul;104(1):239–243. doi: 10.1042/bj1040239

The synthesis of polydeoxyribonucleotide by cell-free extracts of embryonic mouse tissue

G D Birnie 1, Sylvia M Fox 1
PMCID: PMC1270566  PMID: 4291997

Abstract

The conditions under which soluble extracts prepared from mouse embryos incorporate [3H]thymidine 5′-triphosphate into polydeoxyribonucleotide have been studied. In common with similar preparations from other mammalian tissues, mouse-embryo DNA nucleotidyltransferase requires the four complementary deoxyribonucleoside 5′-triphosphates, primer DNA and a bivalent cation for activity. Unlike other mammalian DNA nucleotidyltransferases, the rate and extent of the incorporation of [3H]thymidine 5′-triphosphate is much greater with Mn2+ than with Mg2+ and, with either Mg2+ or Mn2+, maximum activity occurs at pH6·4. The difference between Mg2+ and Mn2+ varies markedly with pH, reaching a maximum of six- to eight-fold at pH6·4.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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