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. 1967 Jul;104(1):270–277. doi: 10.1042/bj1040270

The exchange of unesterified cholesterol between human low-density lipoproteins and rat erythrocyte `ghosts'

K R Bruckdorfer 1, C Green 1
PMCID: PMC1270572  PMID: 6035517

Abstract

1. The exchange of unesterified cholesterol molecules between rat erythrocyte `ghosts' and human low-density lipoproteins has been studied under a number of different experimental conditions. 2. The process is pH-dependent, the rate being minimal at about pH5. 3. Cholesterol exchange does not vary greatly with temperature, the rate at 50° being less than twice that at 2°. 4. Large variations in the ionic strength or Ca2+ concentration of the medium have little effect, but the exchange rate is greatly increased in the presence of a wide range of chemical compounds, e.g. urea, alcohols, acetone, dimethyl sulphoxide and tetra-alkyl-ammonium salts. 5. Acetone and dimethyl sulphoxide have a much greater effect at 37° than at 8–10°. 6. It is proposed that hydrophobic bonding is of great importance in maintaining the structure of `ghosts' and lipoproteins. 7. The results are discussed in relation to current theories of membrane and lipoprotein structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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