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. 1967 Jul;104(1):293–295. doi: 10.1042/bj1040293

A simple procedure for the estimation of very small amounts of nitrogen in lipids

G H Sloane-Stanley 1
PMCID: PMC1270575  PMID: 4291999

Abstract

1. About 0·1μg.atom of combined nitrogen, in lipids and a few other compounds, can be determined quantitatively by the gentle digestion of dry samples in 10ml. test tubes with perchloric acid for 30min., followed by the estimation of the resulting ammonia as the stable blue colour (λmax. 635mμ) produced by the addition of phenol and nitroprusside, an alkaline phosphate buffer and alkaline hypochlorite. 2. Deionized distilled water is required, but the other reagents need no special purification if chosen and handled with care. 3. The results are linear with from 0·015 to 0·15μg.atom of nitrogen, or up to 1μg.atom if the final solutions are diluted with water after full colour development.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett H. S. Polytetrafluoroethylene for the Prevention of Bumping From Superheating of Boiling Fluids. Science. 1947 Dec 26;106(2765):646–646. doi: 10.1126/science.106.2765.646-a. [DOI] [PubMed] [Google Scholar]
  2. CHANEY A. L., MARBACH E. P. Modified reagents for determination of urea and ammonia. Clin Chem. 1962 Apr;8:130–132. [PubMed] [Google Scholar]
  3. EXLEY D. The determination of 20-100 mu mg. quantities of organic nitrogen. Biochem J. 1956 Jul;63(3):496–501. doi: 10.1042/bj0630496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FAWCETT J. K., SCOTT J. E. A rapid and precise method for the determination of urea. J Clin Pathol. 1960 Mar;13:156–159. doi: 10.1136/jcp.13.2.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GATT S., BERMAN E. R. Studies on brain lipids in Tay-Sachs' disease. I. Isolation of two sialic acid-free glycolipids. J Neurochem. 1963 Jan;10:43–49. doi: 10.1111/j.1471-4159.1963.tb05036.x. [DOI] [PubMed] [Google Scholar]
  6. GATT S., BERMAN E. R. Studies on brain lipids in Tay-Sachs' disease. III. Solubility properties of gangliosides. J Neurochem. 1963 Feb;10:65–72. [PubMed] [Google Scholar]
  7. GOTTFRIED S. P., ERDMAN G. L. Use of perchloric acid as a protein precipitant in the determination of acid-soluble phosphates and nonprotein nitrogen in the blood. Am J Clin Pathol. 1951 Feb;21(2):118–122. doi: 10.1093/ajcp/21.2.118. [DOI] [PubMed] [Google Scholar]
  8. HAMILTON P. B. AMINO-ACIDS ON HANDS. Nature. 1965 Jan 16;205:284–285. doi: 10.1038/205284b0. [DOI] [PubMed] [Google Scholar]
  9. KALLER H. [Preparative production of sphingosinephosphorvlcholine]. Biochem Z. 1961;334:451–456. [PubMed] [Google Scholar]
  10. King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LEJEUNE N., THINES-SEMPOUX D., HERS H. G. Tissue fractionation studies. 16. Intracellular distribution and properties of alpha-glucosidases in rat liver. Biochem J. 1963 Jan;86:16–21. doi: 10.1042/bj0860016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LUBOCHINSKY B., ZALTA J. P. Microdosage colorimétrique de l'azote ammoniacal. Bull Soc Chim Biol (Paris) 1954;36(9):1363–1366. [PubMed] [Google Scholar]
  13. Long C., Staples D. A. Chromatographic separation of brain lipids: cerebroside and sulphatide. Biochem J. 1961 Jan;78(1):179–185. doi: 10.1042/bj0780179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MARINETTI G. V., ERBLAND J., KOCHEN J. Quantitative chromatography of phosphatides. Fed Proc. 1957 Sep;16(3):837–844. [PubMed] [Google Scholar]
  15. ROSEVEAR J. W., SMITH E. L. Glycopeptides. I. Isolation and properties of glycopeptides from a fraction of human gamma-globulin. J Biol Chem. 1961 Feb;236:425–435. [PubMed] [Google Scholar]
  16. SLOANE-STANLEY G. H. Anaerobic reactions of phospholipins in brain suspensions. Biochem J. 1953 Mar;53(4):613–619. doi: 10.1042/bj0530613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Searcy R. L., Simms N. M., Foreman J. A., Bergquist L. M. A study of the specificity of the Berthelot colour reaction. Clin Chim Acta. 1965 Aug;12(2):170–175. doi: 10.1016/0009-8981(65)90026-4. [DOI] [PubMed] [Google Scholar]

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