Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Aug;104(2):695–704. doi: 10.1042/bj1040695

The calcium-stimulated incorporation of ethanolamine and serine into the phospholipids of the housefly Musca domestica

H D Crone 1
PMCID: PMC1270639  PMID: 6048809

Abstract

1. The calcium-stimulated incorporation of [2-14C]ethanolamine and l-[3-14C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [32P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. These results are compared with results published for similar mammalian systems, and their possible physiological significance is discussed.

Full text

PDF
695

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRIDGES R. G., RICKETTS J., COX J. T. THE REPLACEMENT OF LIPID-BOUND CHOLINE BY OTHER BASES IN THE PHOSPHOLIPIDS OF THE HOUSEFLY, MUSCA DOMESTICA. J Insect Physiol. 1965 Mar;11:225–236. doi: 10.1016/0022-1910(65)90070-3. [DOI] [PubMed] [Google Scholar]
  2. Bachur N. R., Udenfriend S. Microsomal synthesis of fatty acid amides. J Biol Chem. 1966 Mar 25;241(6):1308–1313. [PubMed] [Google Scholar]
  3. Bjornstad P. Phospholipase activity in rat-liver microsomes studied by the use of endogenous substrates. Biochim Biophys Acta. 1966 Jun 1;116(3):500–510. [PubMed] [Google Scholar]
  4. Brooks G. T., Harrison A. Metabolism of aldrin and dihydroaldrin by houseflies (M. Domestica L.) in vivo and by housefly and pig liver microsomes. Life Sci. 1966 Dec;5(24):2315–2320. doi: 10.1016/0024-3205(66)90068-3. [DOI] [PubMed] [Google Scholar]
  5. CRONE H. D., BRIDGES R. G. THE PHOSPHOLIPIDS OF THE HOUSEFLY, MUSCA DOMESTICA. Biochem J. 1963 Oct;89:11–21. doi: 10.1042/bj0890011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crone H. D. The relationship between phosphatide serine and ethanolamine in larvae of the housefly, Musca domestica. J Insect Physiol. 1967 Jan;13(1):81–90. doi: 10.1016/0022-1910(67)90005-4. [DOI] [PubMed] [Google Scholar]
  7. Cronehd, Newburgh R. W., Mezei C. The larval fat body of Sarcophaga bullata (Diptera) as a system for studying phospholipid biosynthesis. J Insect Physiol. 1966 Jun;12(6):619–624. doi: 10.1016/0022-1910(66)90107-7. [DOI] [PubMed] [Google Scholar]
  8. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DILS R. R., HUBSCHER G. Metabolism of phospholipids. III. The effect of calcium ions on the incorporation of labelled choline into rat-liver microsomes. Biochim Biophys Acta. 1961 Jan 29;46:505–513. doi: 10.1016/0006-3002(61)90581-9. [DOI] [PubMed] [Google Scholar]
  10. HUBSCHER G. Metabolism of phospholipids. VI. The effect of metal ions on the incorporation of L-serine into phosphatidylserine. Biochim Biophys Acta. 1962 Mar 12;57:555–561. doi: 10.1016/0006-3002(62)91163-0. [DOI] [PubMed] [Google Scholar]
  11. KENNEDY E. P., WEISS S. B. The function of cytidine coenzymes in the biosynthesis of phospholipides. J Biol Chem. 1956 Sep;222(1):193–214. [PubMed] [Google Scholar]
  12. MAHLER H. R. The use of amine buffers in studies with enzymes. Ann N Y Acad Sci. 1961 Jun 17;92:426–439. doi: 10.1111/j.1749-6632.1961.tb44992.x. [DOI] [PubMed] [Google Scholar]
  13. Sheltawy A., Dawson R. M. The polyphosphoinositides and other lipids of peripheral nerves. Biochem J. 1966 Jul;100(1):12–18. doi: 10.1042/bj1000012. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES