Abstract
1. Culture filtrates from Trichoderma viride have been fractionated by gel filtration on Sephadex G-75 followed by ion-exchange chromatography on DEAE- and SE-Sephadex. 2. The components essential for attack on cotton are a carboxymethylcellulase, a cellobiase and a third (C1) component which has no action on CM-cellulose, cellobiose or cotton. 3. These components, which together can completely convert cotton into water-soluble products, lose this ability when separated and regain it quantitatively when recombined in their original proportions.
Full text
PDF








Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BROOME J. A RAPID METHOD OF DISK ELECTROPHORESIS. Nature. 1963 Jul 13;199:179–180. doi: 10.1038/199179b0. [DOI] [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- IWASAKI T., HAYASHI K., FUNATSU M. PURIFICATION AND CHARACTERIZATION OF TWO TYPES OF CELLULASE FROM TRICHODERMA KONINGI. J Biochem. 1964 Feb;55:209–212. [PubMed] [Google Scholar]
- Li L. H., Flora R. M., King K. W. Individual roles of cellulase components derived from Trichoderma viride. Arch Biochem Biophys. 1965 Aug;111(2):439–447. doi: 10.1016/0003-9861(65)90207-9. [DOI] [PubMed] [Google Scholar]
- PORATH J., BENNICH H. Recycling chromatography. Arch Biochem Biophys. 1962 Sep;Suppl 1:152–156. [PubMed] [Google Scholar]
- REESE E. T., SIU R. G. H., LEVINSON H. S. The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis. J Bacteriol. 1950 Apr;59(4):485–497. doi: 10.1128/jb.59.4.485-497.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SELBY K., MAITLAND C. C. THE FRACTIONATION OF MYROTHECIUM VERRUCARIA CELLULASE BY GEL FILTRATION. Biochem J. 1965 Mar;94:578–583. doi: 10.1042/bj0940578. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SELBY K. The degradation of cotton cellulose by the extracellular cellulas cellulase of Myrothecium verrucaria. Biochem J. 1961 Jun;79:562–566. doi: 10.1042/bj0790562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selby K., Maitland C. C., Thompson K. V. The degradation of cotton cellulose by the extracellular cellulase of Myrothecium verrucaria. 2. The existence of an ;exhaustible' cellulase. Biochem J. 1963 Aug;88(2):288–296. doi: 10.1042/bj0880288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIMER H. E., MOSHIN J. R. Serum glyco protein concentrations in experimental tuberculosis of guinea pigs. Am Rev Tuberc. 1953 Oct;68(4):594–602. doi: 10.1164/art.1953.68.4.594. [DOI] [PubMed] [Google Scholar]